Nanoscale hybrid implant surfaces and Osterix-mediated osseointegration

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCase Western Reserve University-
Autor(es): dc.contributorUniversity of Michigan-
Autor(es): dc.contributorUniversity of California-
Autor(es): dc.contributorUniversity of Illinois at Chicago-
Autor(es): dc.creatorMorandini Rodrigues, Laís-
Autor(es): dc.creatorLima Zutin, Elis A.-
Autor(es): dc.creatorSartori, Elisa M.-
Autor(es): dc.creatorRizzante, Fabio A. P.-
Autor(es): dc.creatorMendonça, Daniela B. S.-
Autor(es): dc.creatorKrebsbach, Paul H.-
Autor(es): dc.creatorJepsen, Karl J.-
Autor(es): dc.creatorCooper, Lyndon F.-
Autor(es): dc.creatorVasconcellos, Luana M. R.-
Autor(es): dc.creatorMendonça, Gustavo-
Data de aceite: dc.date.accessioned2025-08-21T23:28:12Z-
Data de disponibilização: dc.date.available2025-08-21T23:28:12Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/jbm.a.37323-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222679-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222679-
Descrição: dc.descriptionEndosseous implant surface topography directly affects adherent cell responses following implantation. The aim of this study was to examine the impact of nanoscale topographic modification of titanium implants on Osterix gene expression since this gene has been reported as key factor for bone formation. Titanium implants with smooth and nanoscale topographies were implanted in the femurs of Osterix-Cherry mice for 1–21 days. Implant integration was evaluated using scanning electron microscopy (SEM) to evaluate cell adhesion on implant surfaces, histology, and nanotomography (NanoCT) to observe and quantify the formed bone-to-implant interface, flow cytometry to quantify of Osterix expressing cells in adjacent tissues, and real-time PCR (qPCR) to quantify the osteoinductive and osteogenic gene expression of the implant-adherent cells. SEM revealed topography-dependent adhesion of cells at early timepoints. NanoCT demonstrated greater bone formation at nanoscale implants and interfacial osteogenesis was confirmed histologically at 7 and 14 days for both smooth and nanosurface implants. Flow cytometry revealed greater numbers of Osterix positive cells in femurs implanted with nanoscale versus smooth implants. Compared to smooth surface implants, nanoscale surface adherent cells expressed higher levels of Osterix (Osx), Alkaline phosphatase (Alp), Paired related homeobox (Prx1), Dentin matrix protein 1 (Dmp1), Bone sialoprotein (Bsp), and Osteocalcin (Ocn). In conclusion, nanoscale surface implants demonstrated greater bone formation associated with higher levels of Osterix expression over the 21-day healing period with direct evidence of surface-associated gene regulation involving a nanoscale-mediated osteoinductive pathway that utilizes Osterix to direct adherent cell osteoinduction.-
Descrição: dc.descriptionAcademy of Osseointegration-
Descrição: dc.descriptionInternational Association for Dental Research-
Descrição: dc.descriptionNational Institute of Arthritis and Musculoskeletal and Skin Diseases-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartment of Oral Surgery and Integrated Clinics) School of Dentistry São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartment of Comprehensive Dentistry School of Dental Medicine Case Western Reserve University-
Descrição: dc.descriptionDepartment of Biological and Material Sciences & Prosthodontics School of Dentistry University of Michigan-
Descrição: dc.descriptionSection of Periodontics School of Dentistry University of California-
Descrição: dc.descriptionDepartment of Orthopedic Surgery School of Medicine University of Michigan-
Descrição: dc.descriptionDepartment of Oral Biology College of Dentistry University of Illinois at Chicago-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartment of Oral Surgery and Integrated Clinics) School of Dentistry São Paulo State University (Unesp)-
Descrição: dc.descriptionNational Institute of Arthritis and Musculoskeletal and Skin Diseases: P30 AR069620-
Formato: dc.format696-707-
Idioma: dc.languageen-
Relação: dc.relationJournal of Biomedical Materials Research - Part A-
???dc.source???: dc.sourceScopus-
Título: dc.titleNanoscale hybrid implant surfaces and Osterix-mediated osseointegration-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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