How long does the mRNA remains stable in untreated whole bovine blood?

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorInstituto de Zootecnia-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.creatorGiglioti, Rodrigo-
Autor(es): dc.creatorAzevedo, Bianca Tainá-
Autor(es): dc.creatorde Oliveira, Henrique Nunes-
Autor(es): dc.creatorKatiki, Luciana Morita-
Autor(es): dc.creatorFilho, Anibal Eugênio Vercesi-
Autor(es): dc.creatorde Sena Oliveira, Márcia Cristina-
Autor(es): dc.creatorOkino, Cintia Hiromi-
Data de aceite: dc.date.accessioned2025-08-21T21:06:35Z-
Data de disponibilização: dc.date.available2025-08-21T21:06:35Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s11033-021-06808-w-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222638-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222638-
Descrição: dc.descriptionBackground: High quality and quantity of messenger RNA (mRNA) are required for accuracy of gene expression studies and other RNA-based downstream applications. Since RNA is considered a labile macromolecular prone to degradation, which may result in falsely altered gene expression patterns, several commercial stabilizing reagents have been developed aiming to keep RNA stable for long period. However, for studies involving large number of experimental samples, the high costs related to these specific reagents may constitute a barrier. Methods and results: In this context the present study was designed aiming to evaluate the stability of mRNA in whole bovine blood collected in EDTA tubes during storage at common fridge (4 °C). Whole blood samples were collected from six Holstein calves and submitted to RNA extraction in each different interval: immediately after blood sampling (< 2 h), at 1-day post-sampling (dps), 2 dps, 3 dps, 7 dps and 14dps intervals. RNA integrity and purity were evaluated, and RT-qPCR assays were run using seven different genes (B2M, ACTB, PPIA, GAPDH, YWHAZ, CD4 and IFN-γ) aiming to evaluate the presence of altered gene transcription during storage. All extracted RNA samples presented high purity, while optimal integrity and unaltered gene expression were observed in whole experimental group up to 3 days of storage. Conclusion: Bovine blood RNA remained stable in K3EDTA tubes for 3 days stored at common fridge and can be successfully and accurately used for gene expression studies.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionInstituto de Zootecnia, Rua Heitor Penteado, n. 56,São Paulo-
Descrição: dc.descriptionUniversidade Estadual Júlio de Mesquita Filho Unesp, São Paulo-
Descrição: dc.descriptionEmbrapa Pecuária Sudeste, São Paulo-
Descrição: dc.descriptionUniversidade Estadual Júlio de Mesquita Filho Unesp, São Paulo-
Descrição: dc.descriptionFAPESP: 2016/07216-7-
Descrição: dc.descriptionFAPESP: 2019/22675-6-
Formato: dc.format789-795-
Idioma: dc.languageen-
Relação: dc.relationMolecular Biology Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCattle-
Palavras-chave: dc.subjectFridge-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectRNA integrity-
Palavras-chave: dc.subjectStability-
Palavras-chave: dc.subjectStorage-
Título: dc.titleHow long does the mRNA remains stable in untreated whole bovine blood?-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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