Continuous Synthesis of Biodiesel from Outstanding Kernel Oil in a Packed Bed Reactor Using Burkholderia cepacia Lipase Immobilized on Magnetic Nanosupport

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorEARTH University-
Autor(es): dc.contributorNational Institute for Space Research-
Autor(es): dc.contributorFederal Rural University of Rio de Janeiro-
Autor(es): dc.contributorFederal University of Alfenas-
Autor(es): dc.creatorBento, Heitor B. S.-
Autor(es): dc.creatorReis, Cristiano E. R.-
Autor(es): dc.creatorPinto, Pedro A.-
Autor(es): dc.creatorCortez, Daniela V.-
Autor(es): dc.creatorVilas Bôas, Renata N.-
Autor(es): dc.creatorCosta-Silva, Tales A.-
Autor(es): dc.creatorCarvalho, Ana K. F.-
Autor(es): dc.creatorde Castro, Heizir F.-
Data de aceite: dc.date.accessioned2025-08-21T18:04:19Z-
Data de disponibilização: dc.date.available2025-08-21T18:04:19Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10562-021-03826-y-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222633-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222633-
Descrição: dc.descriptionThis study deals with the use of commercial lipase stabilized onto magnetic particles of iron oxides (Fe3O4/γ-Fe2O3) and applied as a heterogeneous biocatalyst in the synthesis of ethyl esters (biodiesel) in a solvent-free medium on a continuous process. Magnetic particles were synthesized by alkaline co-precipitation, silanized, activated and subsequently used for immobilization of Burkholderia cepacia lipase. The results regarding the lipase immobilization showed an enzyme activity retention of about 53% and an increase in the KM value in about threefold (from 410 to 1262 mmol L−1) and a decrease in the Vmax value in sevenfold (From 12,390 to 1786 U g−1) when compared with the free enzyme. The immobilization process favored the thermal stability and increased the half-time of the enzyme about tenfold at 50 °C. The immobilized derivative was evaluated to aroma production and the activity of esterification was calculated as being 56.7 μmol L−1 g−1 min−1, which corresponds to a productivity value of 0.58 g ester L−1 h−1. The immobilized system was also used to mediate transesterification of kernel oil in a fixed bed reactor operating in a continuous flow with a reaction medium composed of oil and ethanol at a molar ratio of 1:12, 50 °C and space–time of 16 h. The operational stability of the immobilized lipase estimated at 47 days allowed to operate the reactor with high productivity of 38.7 ± 0.7 mg g−1 h−1. Also, the product properties of the ester content (> 96.5%) and kinematic viscosity value (5.32 ± 0.4 mm2 s−1 at 40 °C) meet the requirements of the ANP and ASTM (D6751) for biodiesel fuel. Graphic Abstract: [Figure not available: see fulltext.]-
Descrição: dc.descriptionEngineering School of Lorena University of São Paulo, PO Box 116-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionEARTH University-
Descrição: dc.descriptionNational Institute for Space Research-
Descrição: dc.descriptionFederal Rural University of Rio de Janeiro, Seropédica-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences University of São Paulo-
Descrição: dc.descriptionInstitute of Chemistry Federal University of Alfenas-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationCatalysis Letters-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiodiesel-
Palavras-chave: dc.subjectContinuous reaction-
Palavras-chave: dc.subjectEnzyme immobilization-
Palavras-chave: dc.subjectLipase-
Palavras-chave: dc.subjectMagnetic nanoparticles-
Título: dc.titleContinuous Synthesis of Biodiesel from Outstanding Kernel Oil in a Packed Bed Reactor Using Burkholderia cepacia Lipase Immobilized on Magnetic Nanosupport-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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