Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Paraná-
Autor(es): dc.contributorCentro Universitário Católica de Santa Catarina-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Kentucky-
Autor(es): dc.creatorIantas, Jucélia-
Autor(es): dc.creatorSavi, Daiani Cristina-
Autor(es): dc.creatorSchibelbein, Renata da Silva-
Autor(es): dc.creatorNoriler, Sandriele Aparecida-
Autor(es): dc.creatorAssad, Beatriz Marques-
Autor(es): dc.creatorDilarri, Guilherme-
Autor(es): dc.creatorFerreira, Henrique-
Autor(es): dc.creatorRohr, Jürgen-
Autor(es): dc.creatorThorson, Jon S.-
Autor(es): dc.creatorShaaban, Khaled A.-
Autor(es): dc.creatorGlienke, Chirlei-
Data de aceite: dc.date.accessioned2025-08-21T17:18:26Z-
Data de disponibilização: dc.date.available2025-08-21T17:18:26Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fmicb.2021.714750-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222428-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222428-
Descrição: dc.descriptionPlant diseases caused by phytopathogens are responsible for significant crop losses worldwide. Resistance induction and biological control have been exploited in agriculture due to their enormous potential. In this study, we investigated the antimicrobial potential of endophytic fungi of leaves and petioles of medicinal plants Vochysia divergens and Stryphnodendron adstringens located in two regions of high diversity in Brazil, Pantanal, and Cerrado, respectively. We recovered 1,304 fungal isolates and based on the characteristics of the culture, were assigned to 159 phenotypes. One isolate was selected as representative of each phenotype and studied for antimicrobial activity against phytopathogens. Isolates with better biological activities were identified based on DNA sequences and phylogenetic analyzes. Among the 159 representative isolates, extracts from 12 endophytes that inhibited the mycelial growth (IG) of Colletotrichum abscissum (≥40%) were selected to expand the antimicrobial analysis. The minimum inhibitory concentrations (MIC) of the extracts were determined against citrus pathogens, C. abscissum, Phyllosticta citricarpa and Xanthomonas citri subsp. citri and the maize pathogen Fusarium graminearum. The highest activity against C. abscissum were from extracts of Pseudofusicoccum stromaticum CMRP4328 (IG: 83% and MIC: 40 μg/mL) and Diaporthe vochysiae CMRP4322 (IG: 75% and MIC: 1 μg/mL), both extracts also inhibited the development of post-bloom fruit drop symptoms in citrus flowers. The extracts were promising in inhibiting the mycelial growth of P. citricarpa and reducing the production of pycnidia in citrus leaves. Among the isolates that showed activity, the genus Diaporthe was the most common, including the new species D. cerradensis described in this study. In addition, high performance liquid chromatography, UV detection, and mass spectrometry and thin layer chromatography analyzes of extracts produced by endophytes that showed high activity, indicated D. vochysiae CMRP4322 and P. stromaticum CMRP4328 as promising strains that produce new bioactive natural products. We report here the capacity of endophytic fungi of medicinal plants to produce secondary metabolites with biological activities against phytopathogenic fungi and bacteria. The description of the new species D. cerradensis, reinforces the ability of medicinal plants found in Brazil to host a diverse group of fungi with biotechnological potential.-
Descrição: dc.descriptionCenter for Computational Sciences, University of Kentucky-
Descrição: dc.descriptionCenter for Clinical and Translational Science, University of Kentucky-
Descrição: dc.descriptionCollege of Dentistry, University of Kentucky-
Descrição: dc.descriptionCenter for Pharmaceutical Research and Innovation, University of Kentucky-
Descrição: dc.descriptionTracy Farmer Institute for Sustainability and the Environment, University of Kentucky-
Descrição: dc.descriptionUniversity of Kentucky-
Descrição: dc.descriptionMarkey Cancer Center, University of Kentucky-
Descrição: dc.descriptionSaha Cardiovascular Research Center, University of Kentucky-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFoundation for the National Institutes of Health-
Descrição: dc.descriptionNational Center for Advancing Translational Sciences-
Descrição: dc.descriptionPostgraduate Program of Microbiology Parasitology and Pathology Department of Pathology Federal University of Paraná-
Descrição: dc.descriptionDepartment of Biomedicine Centro Universitário Católica de Santa Catarina-
Descrição: dc.descriptionPostgraduate Program of Genetics Federal University of Paraná-
Descrição: dc.descriptionDepartment of General and Applied Biology Biosciences Institute State University of São Paulo-
Descrição: dc.descriptionDepartment of Pharmaceutical Sciences College of Pharmacy University of Kentucky-
Descrição: dc.descriptionCenter for Pharmaceutical Research and Innovation College of Pharmacy University of Kentucky-
Descrição: dc.descriptionFAPESP: 2015/50162-2-
Descrição: dc.descriptionCNPq: 309971/2016-0-
Descrição: dc.descriptionCNPq: 424738/2016-3-
Descrição: dc.descriptionFoundation for the National Institutes of Health: CA243529-
Descrição: dc.descriptionCNPq: INCT 465440/2014-2-
Descrição: dc.descriptionCenter for Pharmaceutical Research and Innovation, University of Kentucky: NIH P20 GM130456-
Descrição: dc.descriptionFoundation for the National Institutes of Health: R01 GM115261-
Descrição: dc.descriptionNational Center for Advancing Translational Sciences: UL1TR000117-
Descrição: dc.descriptionNational Center for Advancing Translational Sciences: UL1TR001998-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectendophytes-
Palavras-chave: dc.subjectendophytic fungi-
Palavras-chave: dc.subjectnatural product-
Palavras-chave: dc.subjectphytopathogens-
Palavras-chave: dc.subjectsecondary metabolites-
Título: dc.titleEndophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.