Targeting riboswitches with synthetic small RNAs for metabolic engineering

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorInstitute for Technical Microbiology-
Autor(es): dc.creatorLins, Milca Rachel da Costa Ribeiro [UNESP]-
Autor(es): dc.creatorAmorim, Laura Araujo da Silva [UNESP]-
Autor(es): dc.creatorCorrêa, Graciely Gomes [UNESP]-
Autor(es): dc.creatorPicão, Bruno Willian [UNESP]-
Autor(es): dc.creatorMack, Matthias-
Autor(es): dc.creatorCerri, Marcel Otávio [UNESP]-
Autor(es): dc.creatorPedrolli, Danielle Biscaro [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T22:12:23Z-
Data de disponibilização: dc.date.available2022-08-04T22:12:23Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ymben.2021.09.003-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222399-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222399-
Descrição: dc.descriptionOur growing knowledge of the diversity of non-coding RNAs in natural systems and our deepening knowledge of RNA folding and function have fomented the rational design of RNA regulators. Based on that knowledge, we designed and implemented a small RNA tool to target bacterial riboswitches and activate gene expression (rtRNA). The synthetic rtRNA is suitable for regulation of gene expression both in cell-free and in cellular systems. It targets riboswitches to promote the antitermination folding regardless the cognate metabolite concentration. Therefore, it prevents transcription termination increasing gene expression up to 103-fold. We successfully used small RNA arrays for multiplex targeting of riboswitches. Finally, we used the synthetic rtRNAs to engineer an improved riboflavin producer strain. The easiness to design and construct, and the fact that the rtRNA works as a single genome copy, make it an attractive tool for engineering industrial metabolite-producing strains.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) School of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology, Rodovia Araraquara-Jau Km1-
Descrição: dc.descriptionMannheim University of Applied Sciences Institute for Technical Microbiology, Paul-Wittsack-Str. 10-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) School of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology, Rodovia Araraquara-Jau Km1-
Descrição: dc.descriptionFAPESP: 2014/17564-7-
Descrição: dc.descriptionFAPESP: 2020/08699-7-
Descrição: dc.descriptionCNPq: 290110/2017-3-
Formato: dc.format59-67-
Idioma: dc.languageen-
Relação: dc.relationMetabolic Engineering-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBacillus subtilis-
Palavras-chave: dc.subjectNon-coding RNA-
Palavras-chave: dc.subjectRiboflavin-
Palavras-chave: dc.subjectRiboswitch-
Palavras-chave: dc.subjectSmall RNA-
Palavras-chave: dc.subjectSynthetic biology-
Título: dc.titleTargeting riboswitches with synthetic small RNAs for metabolic engineering-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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