FIRST REPORT OF GENUS Cryptosporidium IN CERVIDS SPECIES: Mazama americana, Mazama nana AND Blastocerus dichotomus

Registro completo de metadados
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Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPires Teixeira, Weslen Fabricio-
Autor(es): dc.creatorde Oliveira, Márcio Leite [UNESP]-
Autor(es): dc.creatorde Faria Peres, Pedro Henrique [UNESP]-
Autor(es): dc.creatorMiranda Oliveira, Bruno César [UNESP]-
Autor(es): dc.creatorNagata, Walter Bertequini [UNESP]-
Autor(es): dc.creatorda Silva Vieira, Dielson [UNESP]-
Autor(es): dc.creatorde Andrade Junior, Anassilton Moreira-
Autor(es): dc.creatorDomingos Ferrari, Elis [UNESP]-
Autor(es): dc.creatorBarbanti Duarte, José Maurício [UNESP]-
Autor(es): dc.creatorVasconcelos Meireles, Marcelo [UNESP]-
Autor(es): dc.creatorZanetti Lopes, Welber Daniel-
Autor(es): dc.creatorSaraiva Bresciani, Katia Denise [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T22:12:20Z-
Data de disponibilização: dc.date.available2022-08-04T22:12:20Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s11259-021-09828-w-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222380-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222380-
Descrição: dc.descriptionWe analyzed Cryptosporidium spp. in fecal samples of wild cervids (Ozotoceros bezoarticus, Blastocerus dichotomus, Mazama nana, Mazama americana, and Mazama bororo) from many Brazilian regions, a fact unprecedented in the literature. Sniffer dogs were used to collect 936 fecal samples of cervids from 14 Brazilian localities. Cervids species were identified using polymerase chain reaction (PCR) performed from genomic DNA extracted from 563 fecal samples of Ozotoceros bezoarticus, Blastocerus dichotomus, Mazama nana, Mazama americana, and Mazama bororo. Cryptosporidium spp. oocyst screening was performed using malachite green negative staining. Nested PCR (nPCR) protocols targeting the 18S rRNA and GP60 genes followed by genetic sequencing were performed for Cryptosporidium spp. detection and Cryptosporidium parvum subtyping, respectively. Nested PCR targeting actin gene and genetic sequencing were performed in samples with non-identified Cryptosporidium species by 18S rRNA amplicon sequencing. The association between the occurrence of Cryptosporidium and the presence of bovines in the same locality was evaluated using Fisher’s exact test. The positivity rates of diagnostic methods were compared by McNemar test and the Kappa correlation coefficient. The prevalence rates of Cryptosporidium spp. in cervids were 1.42% (8/563) and 0.36% (2/563) by nPCR and malachite green negative staining, respectively. C. parvum IIaA16G3R1 isolate was identified in three fecal samples from M. americana, two from M. nana and one from B. dichotomus. Cryptosporidium ryanae were found in one sample from B. dichotomus. We identified a new Cryptosporidium genotype, named Cryptosporidium deer genotype BR, from one M. americana fecal sample.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Veterinary Medicine Federal University of Goiás (UFG), Goiás-
Descrição: dc.descriptionSchool of Agricultural and Veterinarian Sciences Sao Paulo State University (UNESP), Sao Paulo-
Descrição: dc.descriptionDepartment of Support Production and Animal Health School of Veterinary Medicine Sao Paulo State University (UNESP), Sao Paulo-
Descrição: dc.descriptionDepartment of Clinic Surgery and Animal Reproduction School of Veterinary Medicine Sao Paulo State University (UNESP), Sao Paulo-
Descrição: dc.descriptionSchool of Agricultural and Veterinarian Sciences Sao Paulo State University (UNESP), Sao Paulo-
Descrição: dc.descriptionDepartment of Support Production and Animal Health School of Veterinary Medicine Sao Paulo State University (UNESP), Sao Paulo-
Descrição: dc.descriptionDepartment of Clinic Surgery and Animal Reproduction School of Veterinary Medicine Sao Paulo State University (UNESP), Sao Paulo-
Descrição: dc.descriptionFAPESP: #10/50748-3-
Descrição: dc.descriptionFAPESP: #12/50206-1-
Descrição: dc.descriptionFAPESP: #15/10086-5-
Descrição: dc.descriptionFAPESP: #15/25742-5-
Descrição: dc.descriptionCNPq: #304456/2016-0-
Descrição: dc.descriptionCNPq: #407777/2016-
Formato: dc.format49-58-
Idioma: dc.languageen-
Relação: dc.relationVeterinary Research Communications-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCoccid-
Palavras-chave: dc.subjectCryptosporidium ryanae-
Palavras-chave: dc.subjectDeer-
Palavras-chave: dc.subjectOocysts-
Palavras-chave: dc.subjectSpecies-
Palavras-chave: dc.subjectWildlife-
Título: dc.titleFIRST REPORT OF GENUS Cryptosporidium IN CERVIDS SPECIES: Mazama americana, Mazama nana AND Blastocerus dichotomus-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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