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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Federal de Goiás (UFG) | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (UNESP) | - |
Autor(es): dc.creator | Pires Teixeira, Weslen Fabricio | - |
Autor(es): dc.creator | de Oliveira, Márcio Leite [UNESP] | - |
Autor(es): dc.creator | de Faria Peres, Pedro Henrique [UNESP] | - |
Autor(es): dc.creator | Miranda Oliveira, Bruno César [UNESP] | - |
Autor(es): dc.creator | Nagata, Walter Bertequini [UNESP] | - |
Autor(es): dc.creator | da Silva Vieira, Dielson [UNESP] | - |
Autor(es): dc.creator | de Andrade Junior, Anassilton Moreira | - |
Autor(es): dc.creator | Domingos Ferrari, Elis [UNESP] | - |
Autor(es): dc.creator | Barbanti Duarte, José Maurício [UNESP] | - |
Autor(es): dc.creator | Vasconcelos Meireles, Marcelo [UNESP] | - |
Autor(es): dc.creator | Zanetti Lopes, Welber Daniel | - |
Autor(es): dc.creator | Saraiva Bresciani, Katia Denise [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-08-04T22:12:20Z | - |
Data de disponibilização: dc.date.available | 2022-08-04T22:12:20Z | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-01-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1007/s11259-021-09828-w | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/222380 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/222380 | - |
Descrição: dc.description | We analyzed Cryptosporidium spp. in fecal samples of wild cervids (Ozotoceros bezoarticus, Blastocerus dichotomus, Mazama nana, Mazama americana, and Mazama bororo) from many Brazilian regions, a fact unprecedented in the literature. Sniffer dogs were used to collect 936 fecal samples of cervids from 14 Brazilian localities. Cervids species were identified using polymerase chain reaction (PCR) performed from genomic DNA extracted from 563 fecal samples of Ozotoceros bezoarticus, Blastocerus dichotomus, Mazama nana, Mazama americana, and Mazama bororo. Cryptosporidium spp. oocyst screening was performed using malachite green negative staining. Nested PCR (nPCR) protocols targeting the 18S rRNA and GP60 genes followed by genetic sequencing were performed for Cryptosporidium spp. detection and Cryptosporidium parvum subtyping, respectively. Nested PCR targeting actin gene and genetic sequencing were performed in samples with non-identified Cryptosporidium species by 18S rRNA amplicon sequencing. The association between the occurrence of Cryptosporidium and the presence of bovines in the same locality was evaluated using Fisher’s exact test. The positivity rates of diagnostic methods were compared by McNemar test and the Kappa correlation coefficient. The prevalence rates of Cryptosporidium spp. in cervids were 1.42% (8/563) and 0.36% (2/563) by nPCR and malachite green negative staining, respectively. C. parvum IIaA16G3R1 isolate was identified in three fecal samples from M. americana, two from M. nana and one from B. dichotomus. Cryptosporidium ryanae were found in one sample from B. dichotomus. We identified a new Cryptosporidium genotype, named Cryptosporidium deer genotype BR, from one M. americana fecal sample. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
Descrição: dc.description | Department of Veterinary Medicine Federal University of Goiás (UFG), Goiás | - |
Descrição: dc.description | School of Agricultural and Veterinarian Sciences Sao Paulo State University (UNESP), Sao Paulo | - |
Descrição: dc.description | Department of Support Production and Animal Health School of Veterinary Medicine Sao Paulo State University (UNESP), Sao Paulo | - |
Descrição: dc.description | Department of Clinic Surgery and Animal Reproduction School of Veterinary Medicine Sao Paulo State University (UNESP), Sao Paulo | - |
Descrição: dc.description | School of Agricultural and Veterinarian Sciences Sao Paulo State University (UNESP), Sao Paulo | - |
Descrição: dc.description | Department of Support Production and Animal Health School of Veterinary Medicine Sao Paulo State University (UNESP), Sao Paulo | - |
Descrição: dc.description | Department of Clinic Surgery and Animal Reproduction School of Veterinary Medicine Sao Paulo State University (UNESP), Sao Paulo | - |
Descrição: dc.description | FAPESP: #10/50748-3 | - |
Descrição: dc.description | FAPESP: #12/50206-1 | - |
Descrição: dc.description | FAPESP: #15/10086-5 | - |
Descrição: dc.description | FAPESP: #15/25742-5 | - |
Descrição: dc.description | CNPq: #304456/2016-0 | - |
Descrição: dc.description | CNPq: #407777/2016 | - |
Formato: dc.format | 49-58 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Veterinary Research Communications | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Coccid | - |
Palavras-chave: dc.subject | Cryptosporidium ryanae | - |
Palavras-chave: dc.subject | Deer | - |
Palavras-chave: dc.subject | Oocysts | - |
Palavras-chave: dc.subject | Species | - |
Palavras-chave: dc.subject | Wildlife | - |
Título: dc.title | FIRST REPORT OF GENUS Cryptosporidium IN CERVIDS SPECIES: Mazama americana, Mazama nana AND Blastocerus dichotomus | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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