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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (UNESP) | - |
Autor(es): dc.contributor | Wageningen University and Research | - |
Autor(es): dc.creator | Cardozo, Marita Vedovelli [UNESP] | - |
Autor(es): dc.creator | Liakopoulos, Apostolos | - |
Autor(es): dc.creator | Brouwer, Michael | - |
Autor(es): dc.creator | Kant, Arie | - |
Autor(es): dc.creator | Pizauro, Lucas José Luduvério [UNESP] | - |
Autor(es): dc.creator | Borzi, Mariana Monezi [UNESP] | - |
Autor(es): dc.creator | Mevius, Dik | - |
Autor(es): dc.creator | de Ávila, Fernando Antonio [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-08-04T22:12:19Z | - |
Data de disponibilização: dc.date.available | 2022-08-04T22:12:19Z | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2021-06-22 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.3389/fmicb.2021.628738 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/222376 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/222376 | - |
Descrição: dc.description | This study aimed to investigate the phylogenetic diversity and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae from chicken, chicken meat, and human clinical isolates in Sao Paolo, Brazil, and characterize their respective ESBL-encoding plasmids. Three hundred samples from chicken cloaca, chicken meat, and clinical isolates were phenotypically and genotypically assessed for ESBL resistance. Isolates were identified by MALDI TOF-MS and further characterized by MLST analysis and phylogenetic grouping. ESBL genes were characterized and their location was determined by I-Ceu-I-PFGE and Southern blot, conjugation, transformation, and PCR-based replicon typing experiments. Thirty-seven ESBL-producing isolates (28 E. coli and 9 K. pneumoniae) that were positive for the blaCTX–M–1 or blaCTX–M–2 gene groups were obtained. Two isolates were negative in the transformation assay, and the chromosomal location of the genes was deduced by Southern blot. The blaCTX–M genes identified were carried on plasmid replicon-types X1, HI2, N, FII-variants, I1 and R. The E. coli isolates belonged to nine sequence types, while the K. pneumoniae isolates belonged to four sequence types. The E. coli isolates belonged to phylotype classification groups A, B1, D, and F. This study demonstrated that isolates from cloacal swabs, chicken meat, and human feces had genetic diversity, with a high frequency of blaCTX–M–15 among chickens, chicken meat, and human feces. Thus, this reinforces the hypothesis that chickens, as well as their by-products, could be an important source of transmission for ESBL-producing pathogens to humans in South America. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Department of Veterinary Pathology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp) | - |
Descrição: dc.description | Department of Bacteriology and Epidemiology Wageningen Bioveterinary Research Wageningen University and Research | - |
Descrição: dc.description | Department of Tecnology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp) | - |
Descrição: dc.description | Department of Veterinary Pathology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp) | - |
Descrição: dc.description | Department of Tecnology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp) | - |
Descrição: dc.description | FAPESP: 2013/18280-0 | - |
Descrição: dc.description | FAPESP: 2015/10140-0 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Frontiers in Microbiology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | antibiotic resistance | - |
Palavras-chave: dc.subject | extended spectrum beta lactamases (ESBLs) | - |
Palavras-chave: dc.subject | food chain | - |
Palavras-chave: dc.subject | plasmids | - |
Palavras-chave: dc.subject | poultry | - |
Título: dc.title | Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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