Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Roraima (UFRR)-
Autor(es): dc.contributor(CeTICs/FAPESP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorButantan Institute-
Autor(es): dc.creatorEto, Silas Fernandes-
Autor(es): dc.creatorFernandes, Dayanne Carla-
Autor(es): dc.creatorBaldassi, Amanda Cristina [UNESP]-
Autor(es): dc.creatorBalbuena, Thiago Santana [UNESP]-
Autor(es): dc.creatorda Costa Alecrim, João Victor-
Autor(es): dc.creatorAlmeida de Carvalho, Fabíola Christian-
Autor(es): dc.creatorLima, Carla-
Autor(es): dc.creatorLopes-Ferreira, Monica-
Autor(es): dc.creatorPizauro, João Martins [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T22:12:08Z-
Data de disponibilização: dc.date.available2022-08-04T22:12:08Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.fsi.2021.08.019-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222324-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222324-
Descrição: dc.descriptionGroup B Streptococcus (GBS) causes meningitis in neonates and Nile tilapia (Oreochromis niloticus). The molecular mechanisms regulating the intracellular survival of this pathogen in the host cell are complex and crucial for the progression of infection. Thus, we propose the use of GBS-infected Nile tilapia microglia as an in vitro model system simulating infection caused by homologous bacteria in humans. We used this model to evaluate the phagocytic activity, as well as the functional aspects of the capsular proteins A, B, C, and D and the major redox enzymes, and the synergistic role of mechanisms/proteins involved in blocking phagocytic process. We observed that in the intracellular phase, GBS showed enhanced synthesis of the polysaccharide capsule and used superoxide dismutase, thioredoxin, NADH oxidase, and alkyl hydroperoxide reductase to scavenge reactive oxygen species and reactive nitrogen species produced by the host cell. Furthermore, although these virulence mechanisms were effective during the initial hours of infection, they were not able to subvert microglial responses, which partially neutralized the infection. Altogether, our findings provided important information regarding the intracellular survival mechanisms of GBS and perspectives for the production of new drugs and vaccines, through the druggability analysis of specific proteins. In conclusion, tilapia microglia serve as a potent in vitro experimental model for the study of meningitis.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Postgraduate in Health Sciences-PROCISA Federal University of Roraima (UFRR)-
Descrição: dc.descriptionImmunochemistry Laboratory Butantan Institute (CeTICs/FAPESP), Vital Brazil Avenue, 1500-
Descrição: dc.descriptionDepartment of Technology School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp)-
Descrição: dc.descriptionImmunoregulation Unit of the Laboratory of Applied Toxinology (CeTICs/FAPESP) Butantan Institute, Vital Brazil Avenue, 1500-
Descrição: dc.descriptionDepartment of Technology School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp)-
Formato: dc.format34-50-
Idioma: dc.languageen-
Relação: dc.relationFish and Shellfish Immunology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBrain-
Palavras-chave: dc.subjectDruggability-
Palavras-chave: dc.subjectMeningitis-
Palavras-chave: dc.subjectProteomics-
Palavras-chave: dc.subjectVaccine-
Título: dc.titleProteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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