Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorState University of Maranhão Tocantine Region-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Florida-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorOliveira, M. L.-
Autor(es): dc.creatorMello, B. P.-
Autor(es): dc.creatorGonella-Diaza, A. M.-
Autor(es): dc.creatorScolari, S. C.-
Autor(es): dc.creatorPugliesi, G.-
Autor(es): dc.creatorMartins, T.-
Autor(es): dc.creatorFeltrin, I. R. [UNESP]-
Autor(es): dc.creatorSartori, R.-
Autor(es): dc.creatorCanavessi, A. M.O.-
Autor(es): dc.creatorBinelli, M.-
Autor(es): dc.creatorMembrive, C. M.B. [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T22:12:00Z-
Data de disponibilização: dc.date.available2022-08-04T22:12:00Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.domaniend.2021.106653-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222281-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222281-
Descrição: dc.descriptionIn cattle, 17β-estradiol (E2) stimulates prostaglandin F2α (PGF2α) synthesis, which causes luteolysis. Except for the well-established upregulation of oxytocin receptor gene (OXTR), molecular mechanisms of E2-induced PGF2α release in vivo remain unknown. We hypothesized that E2-induced PGF2α release requires de novo transcription of components of the PGF2α synthesis machinery. Beef cows (n = 52) were assigned to remain untreated (Control; n = 10), to receive 50% ethanol infusion intravenously (Placebo; n = 21), or 3 mg E2 in 50% ethanol infusion intravenously (Estradiol; n = 21) on day 15 (D15) after estrus. We collected a single endometrial biopsy per animal at the time of the treatment (0h; Control B0h group), 4 hours (4h; Placebo B4h group and Estradiol B4h group), or 7 hours (7h; Placebo B7h group and Estradiol B7h group) post-treatment. Compared to the Placebo group, the Estradiol group presented significantly greater 13,14-dihydro-15-keto-PGF2α concentrations between 4h and 7h and underwent earlier luteolysis. At 4h, the qPCR analysis showed a lower abundance of ESR1, ESR2 and aldo-keto reductase family 1 member B1 (AKR1B1) genes in the Estradiol B4h group, and a greater abundance of OXTR compared to the Placebo B4h group. Similarly, the E2 treatment significantly reduced the abundance of AKR1B1, and AKR1C4 in the Estradiol B7h group, compared to the placebo group. Overall, E2-induced PGF2α release and luteolysis involved an unexpected and transient downregulation of components of the PGF2α-synthesis cascade, except for OXTR, which was upregulated. Collectively, our data suggest that E2 connects newly-synthesized OXTR to pre-existing cellular machinery to synthesize PGF2α and cause luteal regression.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionAgrarian Sciences Center State University of Maranhão Tocantine Region-
Descrição: dc.descriptionDepartment of Animal Reproduction University of São Paulo-
Descrição: dc.descriptionNorth Florida Research and Education Center Institute of Food and Agricultural Sciences University of Florida-
Descrição: dc.descriptionDepartment of Animal Sciences University of Florida-
Descrição: dc.descriptionDepartment of Pharmacology and Biotechnology São Paulo State University-
Descrição: dc.descriptionDepartment of Animal Science Luiz de Queiroz College of Agriculture University of São Paulo-
Descrição: dc.descriptionDepartment of Animal Sciences São Paulo State University-
Descrição: dc.descriptionDepartment of Pharmacology and Biotechnology São Paulo State University-
Descrição: dc.descriptionDepartment of Animal Sciences São Paulo State University-
Descrição: dc.descriptionFAPESP: 2015/03331-3)-
Idioma: dc.languageen-
Relação: dc.relationDomestic Animal Endocrinology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEndometrium-
Palavras-chave: dc.subjectEstrogen-
Palavras-chave: dc.subjectLuteolysis-
Palavras-chave: dc.subjectProstaglandin-
Título: dc.titleUnravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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