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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Paulista University | - |
Autor(es): dc.contributor | Universidade Estadual de Campinas (UNICAMP) | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (UNESP) | - |
Autor(es): dc.contributor | University of Taubaté | - |
Autor(es): dc.creator | Ferreira, Rogério S. | - |
Autor(es): dc.creator | Assis, Rahyza I. F. | - |
Autor(es): dc.creator | Feltran, Geórgia da S. [UNESP] | - |
Autor(es): dc.creator | do Rosário Palma, Iasmin Caroline | - |
Autor(es): dc.creator | Françoso, Beatriz G. | - |
Autor(es): dc.creator | Zambuzzi, Willian F. [UNESP] | - |
Autor(es): dc.creator | Andia, Denise C. | - |
Autor(es): dc.creator | da Silva, Rodrigo A. | - |
Data de aceite: dc.date.accessioned | 2022-08-04T22:11:44Z | - |
Data de disponibilização: dc.date.available | 2022-08-04T22:11:44Z | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-03-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1002/JPER.21-0218 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/222193 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/222193 | - |
Descrição: dc.description | Background: Mesenchymal cells’ biology has been an important investigative tool to maximize bone regeneration through tissue engineering. Here we used mesenchymal cells from periodontal ligament (PDLCs) with high (h-) and low (l-) osteogenic potential, isolated from different donors, to investigate the impact of the individual epigenetic and transcriptional profiles on the osteogenic potential. Methods: Genome-wide and gene-specific DNA (hydroxy) methylation, mRNA expression and immunofluorescence analysis were carried out in h- and l-PDLCs at DMEM (non-induced to osteogenesis) and OM (induced—3rd and 10th days of osteogenic differentiation) groups in vitro. Results: Genome-wide results showed distinct epigenetic profile among PDLCs with most of the differences on 10th day of OM; DMEMs showed higher concentrations (xOM) of differentially methylated probes in gene body, intronic and open sea (3rd day), increasing this concentration in TSS200 and island regions, at 10 days. At basal levels, h- and l-PDLCs showed different transcriptional profiles; l-PDLCs demonstrated higher levels of NANOG/OCT4/SOX2, BAPX1, DNMT3A, TET1/3, and lower levels of RUNX2 transcripts, confirmed by NANOG/OCT4 and RUNX2 immunofluorescence. After osteogenic induction, the distinct transcriptional profile of multipotentiality genes was maintained among PDLCs. In l-PDLCs, the anti-correlation between DNA methylation and gene expression in RUNX2 and NANOG indicates methylation could play a role in modulating both transcripts. Conclusions: Epigenetic and transcriptional distinct profiles detected at basal levels among PDLCs were maintained after osteogenic induction. We cannot discard the existence of a complex that represses osteogenesis, suggesting the individual donors’ characteristics have significant impact on the osteogenic phenotype acquisition. | - |
Descrição: dc.description | School of Dentistry Health Science Institute Paulista University | - |
Descrição: dc.description | Department of Prosthodontics and Periodontics Piracicaba Dental School University of Campinas | - |
Descrição: dc.description | Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University | - |
Descrição: dc.description | Department of Dentistry University of Taubaté | - |
Descrição: dc.description | Program in Environmental and Experimental Pathology Paulista University | - |
Descrição: dc.description | Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University | - |
Formato: dc.format | 435-448 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Journal of Periodontology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | DNA methylation | - |
Palavras-chave: dc.subject | epigenomics | - |
Palavras-chave: dc.subject | mesenchymal stem cells | - |
Palavras-chave: dc.subject | osteogenesis | - |
Palavras-chave: dc.subject | transcription | - |
Título: dc.title | Genome-wide DNA (hydroxy) methylation reveals the individual epigenetic landscape importance on osteogenic phenotype acquisition in periodontal ligament cells | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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