Phytocystatin CsinCPI-2 Reduces Osteoclastogenesis and Alveolar Bone Loss

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity Medical Center of the Johannes Gutenberg University-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversity of Gothenburg-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.creatorDa Ponte Leguizamón, N. [UNESP]-
Autor(es): dc.creatorde Molon, R. S. [UNESP]-
Autor(es): dc.creatorColetto-Nunes, G. [UNESP]-
Autor(es): dc.creatorNogueira, A. V.B.-
Autor(es): dc.creatorRocha, S. V.-
Autor(es): dc.creatorNeo-Justino, D. M.-
Autor(es): dc.creatorSoares-Costa, A.-
Autor(es): dc.creatorCerri, P. S. [UNESP]-
Autor(es): dc.creatorLerner, U. H.-
Autor(es): dc.creatorSouza, P. P.C.-
Autor(es): dc.creatorCirelli, J. A. [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T22:11:31Z-
Data de disponibilização: dc.date.available2022-08-04T22:11:31Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1177/00220345211027811-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222132-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222132-
Descrição: dc.descriptionPeriodontal disease (PD) is a polymicrobial chronic inflammatory condition of the supporting tissues around the teeth, leading to the destruction of surrounding connective tissue. During the progression of PD, osteoclasts play a crucial role in the resorption of alveolar bone that eventually leads to the loss of teeth if the PD is left untreated. Therefore, the development of antiresorptive therapies targeting bone-resorbing cells will significantly benefit the treatment of PD. Here, we demonstrate the inhibitory effect of CsinCPI-2, a novel cysteine peptidase inhibitor from the orange tree, on periodontitis-induced inflammation, alveolar bone loss, and osteoclast differentiation. Using the ligature-induced periodontitis model in mice, we show that treatment with CsinCPI-2 (0.8 µg/g of body weight) significantly reduced inflammatory cell infiltrate in the connective tissue and prevented the loss of alveolar bone mass (BV/TV) caused by PD, effects associated with diminished numbers of TRAP-positive multinucleated cells. Furthermore, CsinCPI-2 significantly downregulated the numbers of inflammatory cells expressing CD3, CD45, MAC387, and IL-1β. In vitro, CsinCPI-2 inhibited RANKL-induced TRAP+ multinucleated osteoclast formation in mouse bone marrow macrophage cultures in a concentration-dependent manner. This effect was not due to cytotoxicity, as demonstrated by the MTT assay. CsinCPI-2 inhibited RANKL-induced mRNA expression of Acp5, Calcr, and Ctsk, as well as the RANKL-induced upregulation of Nfatc1, a crucial transcription factor for osteoclast differentiation. Based on our findings, CsinCPI-2 prevents bone loss induced by PD by controlling the inflammatory process and acting directly on osteoclastogenesis, suggesting an interesting potential for CsinCPI-2 in the strategy for PD treatment.-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University–UNESP-
Descrição: dc.descriptionDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University-
Descrição: dc.descriptionDepartment of Genetics and Evolution Federal University of Sao Carlos-
Descrição: dc.descriptionDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University–UNESP-
Descrição: dc.descriptionCentre for Bone and Arthritis Research Department of Internal Medicine and Clinical Nutrition Institute for Medicine Sahlgrenska Academy University of Gothenburg-
Descrição: dc.descriptionInnovation in Biomaterials Laboratory Faculty of Dentistry Federal University of Goiás-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University–UNESP-
Descrição: dc.descriptionDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University–UNESP-
Formato: dc.format216-225-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dental Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbone resorption-
Palavras-chave: dc.subjectcystatins-
Palavras-chave: dc.subjectinflammation-
Palavras-chave: dc.subjectosteoclasts-
Palavras-chave: dc.subjectperiodontal diseases-
Palavras-chave: dc.subjectperiodontitis-
Título: dc.titlePhytocystatin CsinCPI-2 Reduces Osteoclastogenesis and Alveolar Bone Loss-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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