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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (UNESP) | - |
Autor(es): dc.contributor | University Medical Center of the Johannes Gutenberg University | - |
Autor(es): dc.contributor | Universidade Federal de São Carlos (UFSCar) | - |
Autor(es): dc.contributor | University of Gothenburg | - |
Autor(es): dc.contributor | Universidade Federal de Goiás (UFG) | - |
Autor(es): dc.creator | Da Ponte Leguizamón, N. [UNESP] | - |
Autor(es): dc.creator | de Molon, R. S. [UNESP] | - |
Autor(es): dc.creator | Coletto-Nunes, G. [UNESP] | - |
Autor(es): dc.creator | Nogueira, A. V.B. | - |
Autor(es): dc.creator | Rocha, S. V. | - |
Autor(es): dc.creator | Neo-Justino, D. M. | - |
Autor(es): dc.creator | Soares-Costa, A. | - |
Autor(es): dc.creator | Cerri, P. S. [UNESP] | - |
Autor(es): dc.creator | Lerner, U. H. | - |
Autor(es): dc.creator | Souza, P. P.C. | - |
Autor(es): dc.creator | Cirelli, J. A. [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-08-04T22:11:31Z | - |
Data de disponibilização: dc.date.available | 2022-08-04T22:11:31Z | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-01-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1177/00220345211027811 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/222132 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/222132 | - |
Descrição: dc.description | Periodontal disease (PD) is a polymicrobial chronic inflammatory condition of the supporting tissues around the teeth, leading to the destruction of surrounding connective tissue. During the progression of PD, osteoclasts play a crucial role in the resorption of alveolar bone that eventually leads to the loss of teeth if the PD is left untreated. Therefore, the development of antiresorptive therapies targeting bone-resorbing cells will significantly benefit the treatment of PD. Here, we demonstrate the inhibitory effect of CsinCPI-2, a novel cysteine peptidase inhibitor from the orange tree, on periodontitis-induced inflammation, alveolar bone loss, and osteoclast differentiation. Using the ligature-induced periodontitis model in mice, we show that treatment with CsinCPI-2 (0.8 µg/g of body weight) significantly reduced inflammatory cell infiltrate in the connective tissue and prevented the loss of alveolar bone mass (BV/TV) caused by PD, effects associated with diminished numbers of TRAP-positive multinucleated cells. Furthermore, CsinCPI-2 significantly downregulated the numbers of inflammatory cells expressing CD3, CD45, MAC387, and IL-1β. In vitro, CsinCPI-2 inhibited RANKL-induced TRAP+ multinucleated osteoclast formation in mouse bone marrow macrophage cultures in a concentration-dependent manner. This effect was not due to cytotoxicity, as demonstrated by the MTT assay. CsinCPI-2 inhibited RANKL-induced mRNA expression of Acp5, Calcr, and Ctsk, as well as the RANKL-induced upregulation of Nfatc1, a crucial transcription factor for osteoclast differentiation. Based on our findings, CsinCPI-2 prevents bone loss induced by PD by controlling the inflammatory process and acting directly on osteoclastogenesis, suggesting an interesting potential for CsinCPI-2 in the strategy for PD treatment. | - |
Descrição: dc.description | Department of Diagnosis and Surgery School of Dentistry São Paulo State University–UNESP | - |
Descrição: dc.description | Department of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University | - |
Descrição: dc.description | Department of Genetics and Evolution Federal University of Sao Carlos | - |
Descrição: dc.description | Department of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University–UNESP | - |
Descrição: dc.description | Centre for Bone and Arthritis Research Department of Internal Medicine and Clinical Nutrition Institute for Medicine Sahlgrenska Academy University of Gothenburg | - |
Descrição: dc.description | Innovation in Biomaterials Laboratory Faculty of Dentistry Federal University of Goiás | - |
Descrição: dc.description | Department of Diagnosis and Surgery School of Dentistry São Paulo State University–UNESP | - |
Descrição: dc.description | Department of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University–UNESP | - |
Formato: dc.format | 216-225 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Journal of Dental Research | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | bone resorption | - |
Palavras-chave: dc.subject | cystatins | - |
Palavras-chave: dc.subject | inflammation | - |
Palavras-chave: dc.subject | osteoclasts | - |
Palavras-chave: dc.subject | periodontal diseases | - |
Palavras-chave: dc.subject | periodontitis | - |
Título: dc.title | Phytocystatin CsinCPI-2 Reduces Osteoclastogenesis and Alveolar Bone Loss | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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