Post-treatments of polydopamine coatings influence cellular response

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorAarhus University-
Autor(es): dc.contributorSino-Danish Center for Education and Research-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorDavidsen, Maiken B.-
Autor(es): dc.creatorTeixeira, Jorge Felipe Lima [UNESP]-
Autor(es): dc.creatorDehli, Jeppe-
Autor(es): dc.creatorKarlsson, Christian-
Autor(es): dc.creatorKraft, David-
Autor(es): dc.creatorSouza, Pedro P.C. [UNESP]-
Autor(es): dc.creatorFoss, Morten-
Data de aceite: dc.date.accessioned2022-08-04T22:11:28Z-
Data de disponibilização: dc.date.available2022-08-04T22:11:28Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.colsurfb.2021.111972-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222119-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222119-
Descrição: dc.descriptionPolydopamine (PDA) is the final oxidation product of dopamine or other catecholamines. Since the first reports of PDA coatings starting around 2007, these coatings have been widely studied as a versatile and inexpensive one-step coating option for biomaterial functionalization. The coating attach to a wide range of materials and can subsequently be modified with biomolecules or nanoparticles. However, as a strong candidate for biomaterial research and even clinical use, it is important to unravel the changes in physico-chemical properties and the cell-PDA interaction as a function of heat sterilization procedures and shelf storage periods. Four groups were examined in this study: titanium (Ti), PDA-coated Ti samples and PDA-coated Ti samples either stored for up to two weeks at room temperature or heated at 121 °C for 24 h, respectively. We used X-ray Photoelectron Spectroscopy (XPS), Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) and Water contact angle (WCA) to characterize chemical composition and surface properties of the groups. Cell adhesion and proliferation was examined by three different cell types: human primary dermal fibroblasts (hDF), human epidermal keratinocytes (HaCaTs) and a murine preosteoblastic cell line (MC3T3-E1), respectively. Cells were cultured on PDA coated samples for 4 h, 3 days and 5 days. Both thermal treatment of PDA at 121℃ for 24 h and storage of the samples for 2 weeks increased the amount of quinone groups at the surface and decreased the amount of primary amine groups as detected by XPS and ToF-SIMS. Even though these surface reactions increased the WCA of the PDA coating, we found that the post-treatments increased cell proliferation for both hDFs, HaCaTs and MC3T3-E1 s as compared to pristine PDA. This emphasizes the importance of post-treatment and shelf-time for PDA coatings.-
Descrição: dc.descriptionInnovationsfonden-
Descrição: dc.descriptionInterdisciplinary Nanoscience Center (iNANO) Faculty of Natural Sciences Aarhus University-
Descrição: dc.descriptionSino-Danish Center for Education and Research-
Descrição: dc.descriptionDepartment of Dentistry and Oral Health Faculty of Health Aarhus University-
Descrição: dc.descriptionInnovation in Biomaterials Laboratory (iBioM) School of Dentistry Federal University of Goiás-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationColloids and Surfaces B: Biointerfaces-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCell proliferation-
Palavras-chave: dc.subjectPolydopamine-
Palavras-chave: dc.subjectPost-treatment-
Palavras-chave: dc.subjectSurface functionalization-
Título: dc.titlePost-treatments of polydopamine coatings influence cellular response-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.