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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Aarhus University | - |
Autor(es): dc.contributor | Sino-Danish Center for Education and Research | - |
Autor(es): dc.contributor | Universidade Federal de Goiás (UFG) | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (UNESP) | - |
Autor(es): dc.creator | Davidsen, Maiken B. | - |
Autor(es): dc.creator | Teixeira, Jorge Felipe Lima [UNESP] | - |
Autor(es): dc.creator | Dehli, Jeppe | - |
Autor(es): dc.creator | Karlsson, Christian | - |
Autor(es): dc.creator | Kraft, David | - |
Autor(es): dc.creator | Souza, Pedro P.C. [UNESP] | - |
Autor(es): dc.creator | Foss, Morten | - |
Data de aceite: dc.date.accessioned | 2022-08-04T22:11:28Z | - |
Data de disponibilização: dc.date.available | 2022-08-04T22:11:28Z | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2021-10-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1016/j.colsurfb.2021.111972 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/222119 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/222119 | - |
Descrição: dc.description | Polydopamine (PDA) is the final oxidation product of dopamine or other catecholamines. Since the first reports of PDA coatings starting around 2007, these coatings have been widely studied as a versatile and inexpensive one-step coating option for biomaterial functionalization. The coating attach to a wide range of materials and can subsequently be modified with biomolecules or nanoparticles. However, as a strong candidate for biomaterial research and even clinical use, it is important to unravel the changes in physico-chemical properties and the cell-PDA interaction as a function of heat sterilization procedures and shelf storage periods. Four groups were examined in this study: titanium (Ti), PDA-coated Ti samples and PDA-coated Ti samples either stored for up to two weeks at room temperature or heated at 121 °C for 24 h, respectively. We used X-ray Photoelectron Spectroscopy (XPS), Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) and Water contact angle (WCA) to characterize chemical composition and surface properties of the groups. Cell adhesion and proliferation was examined by three different cell types: human primary dermal fibroblasts (hDF), human epidermal keratinocytes (HaCaTs) and a murine preosteoblastic cell line (MC3T3-E1), respectively. Cells were cultured on PDA coated samples for 4 h, 3 days and 5 days. Both thermal treatment of PDA at 121℃ for 24 h and storage of the samples for 2 weeks increased the amount of quinone groups at the surface and decreased the amount of primary amine groups as detected by XPS and ToF-SIMS. Even though these surface reactions increased the WCA of the PDA coating, we found that the post-treatments increased cell proliferation for both hDFs, HaCaTs and MC3T3-E1 s as compared to pristine PDA. This emphasizes the importance of post-treatment and shelf-time for PDA coatings. | - |
Descrição: dc.description | Innovationsfonden | - |
Descrição: dc.description | Interdisciplinary Nanoscience Center (iNANO) Faculty of Natural Sciences Aarhus University | - |
Descrição: dc.description | Sino-Danish Center for Education and Research | - |
Descrição: dc.description | Department of Dentistry and Oral Health Faculty of Health Aarhus University | - |
Descrição: dc.description | Innovation in Biomaterials Laboratory (iBioM) School of Dentistry Federal University of Goiás | - |
Descrição: dc.description | Department of Physiology and Pathology School of Dentistry São Paulo State University | - |
Descrição: dc.description | Department of Physiology and Pathology School of Dentistry São Paulo State University | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Colloids and Surfaces B: Biointerfaces | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Cell proliferation | - |
Palavras-chave: dc.subject | Polydopamine | - |
Palavras-chave: dc.subject | Post-treatment | - |
Palavras-chave: dc.subject | Surface functionalization | - |
Título: dc.title | Post-treatments of polydopamine coatings influence cellular response | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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