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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Université Laval | - |
Autor(es): dc.contributor | University Laval | - |
Autor(es): dc.contributor | Universidade Estadual de Campinas (UNICAMP) | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (UNESP) | - |
Autor(es): dc.contributor | Dalhousie University | - |
Autor(es): dc.creator | Henaux, Loïc | - |
Autor(es): dc.creator | Pereira, Karina Danielle [UNESP] | - |
Autor(es): dc.creator | Thibodeau, Jacinthe | - |
Autor(es): dc.creator | Pilon, Geneviève | - |
Autor(es): dc.creator | Gill, Tom | - |
Autor(es): dc.creator | Marette, André | - |
Autor(es): dc.creator | Bazinet, Laurent | - |
Data de aceite: dc.date.accessioned | 2022-08-04T22:10:42Z | - |
Data de disponibilização: dc.date.available | 2022-08-04T22:10:42Z | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2021-07-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.3390/membranes11070528 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/222057 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/222057 | - |
Descrição: dc.description | Natural bioactive peptides are suitable candidates for preventing the development of Type 2 diabetes (T2D), by reducing the various risk factors. The aim of this study was to concentrate glucoregulatory and anti-inflammatory peptides, from salmon by-products, by electrodialysis with ultrafiltration membrane (EDUF), and to identify peptides responsible for these bioactivities. Two EDUF configurations (1 and 2) were used to concentrate anionic and cationic peptides, respectively. After EDUF separation, two fractions demonstrated interesting properties: the initial fraction of the EDUF configuration 1 and the final fraction of the EDUF configuration 2 both showed biological activities to (1) increase glucose uptake in L6 muscle cells in insulin condition at 1 ng/mL (by 12% and 21%, respectively), (2) decrease hepatic glucose production in hepatic cells at 1 ng/mL in basal (17% and 16%, respectively), and insulin (25% and 34%, respectively) conditions, and (3) decrease LPS-induced inflammation in macrophages at 1 g/mL (45% and 30%, respectively). More impressive, the initial fraction of the EDUF configuration 1 (45% reduction) showed the same effect as the phenformin at 10 µM (40%), a drug used to treat T2D. Thirteen peptides were identified, chemically synthesized, and tested in-vitro for these three bioactivities. Thus, four new bioactive peptides were identified: IPVE increased glucose uptake by muscle cells, IVDI and IEGTL decreased hepatic glucose production (HGP) of insulin, whereas VAPEEHPTL decreased HGP under both basal condition and in the presence of insulin. To the best of our knowledge, this is the first time that (1) bioactive peptide fractions generated after separation by EDUF were demonstrated to be bioactive on three different criteria; all involved in the T2D, and (2) potential sequences involved in the improvement of glucose uptake and/or in the regulation of HGP were identified from a salmon protein hydrolysate. | - |
Descrição: dc.description | Canadian Institutes of Health Research | - |
Descrição: dc.description | Department of Food Sciences and Laboratory of Food Processing and Electromembrane Processes (LTAPEM) Université Laval | - |
Descrição: dc.description | Institute of Nutrition and Functional Foods (INAF) University Laval | - |
Descrição: dc.description | Laboratory of Biotechnology School of Applied Sciences University of Campinas (UNICAMP) | - |
Descrição: dc.description | Institute of Biosciences State University (UNESP) | - |
Descrição: dc.description | Department of Medicine Faculty of Medicine Quebec Heart and Lung Institute Cardiology Group Université Laval, 2725 Chemin Ste-Foy | - |
Descrição: dc.description | Department of Process Engineering and Applied Science Dalhousie University, P.O. Box 15000 | - |
Descrição: dc.description | Institute of Biosciences State University (UNESP) | - |
Descrição: dc.description | Canadian Institutes of Health Research: FH4-129922 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Membranes | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Anti-inflammatory activity | - |
Palavras-chave: dc.subject | Bioactive peptides | - |
Palavras-chave: dc.subject | Electrodialysis with filtration membrane | - |
Palavras-chave: dc.subject | Glucose uptake | - |
Palavras-chave: dc.subject | Hepatic glucose production | - |
Palavras-chave: dc.subject | Salmon protein hydrolysate | - |
Título: dc.title | Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptides | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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