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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Rutgers Cancer Institute of New Jersey | - |
Autor(es): dc.contributor | Rutgers New Jersey Medical School | - |
Autor(es): dc.contributor | Rice University | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (UNESP) | - |
Autor(es): dc.contributor | Harvard Medical School | - |
Autor(es): dc.contributor | Rutgers Robert Wood Johnson Medical School | - |
Autor(es): dc.contributor | Northeastern University | - |
Autor(es): dc.contributor | State University of New Jersey | - |
Autor(es): dc.contributor | University of California San Diego | - |
Autor(es): dc.creator | Staquicini, Daniela I. | - |
Autor(es): dc.creator | Tang, Fenny H.F. | - |
Autor(es): dc.creator | Markosian, Christopher | - |
Autor(es): dc.creator | Yao, Virginia J. | - |
Autor(es): dc.creator | Staquicini, Fernanda I. | - |
Autor(es): dc.creator | Dodero-Rojas, Esteban | - |
Autor(es): dc.creator | Contessoto, Vinícius G. [UNESP] | - |
Autor(es): dc.creator | Davis, Deodate | - |
Autor(es): dc.creator | O’Brien, Paul | - |
Autor(es): dc.creator | Habib, Nazia | - |
Autor(es): dc.creator | Smith, Tracey L. | - |
Autor(es): dc.creator | Bruiners, Natalie | - |
Autor(es): dc.creator | Sidman, Richard L. | - |
Autor(es): dc.creator | Gennaro, Maria L. | - |
Autor(es): dc.creator | Lattime, Edmund C. | - |
Autor(es): dc.creator | Libutti, Steven K. | - |
Autor(es): dc.creator | Whitford, Paul C. | - |
Autor(es): dc.creator | Burley, Stephen K. | - |
Autor(es): dc.creator | Onuchic, José N. | - |
Autor(es): dc.creator | Arap, Wadih | - |
Autor(es): dc.creator | Pasqualini, Renata | - |
Data de aceite: dc.date.accessioned | 2022-08-04T22:10:35Z | - |
Data de disponibilização: dc.date.available | 2022-08-04T22:10:35Z | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2021-07-27 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1073/pnas.2105739118 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/222021 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/222021 | - |
Descrição: dc.description | Development of effective vaccines against coronavirus disease 2019 (COVID-19) is a global imperative. Rapid immunization of the entire human population against a widespread, continually evolving, and highly pathogenic virus is an unprecedented challenge, and different vaccine approaches are being pursued. Engineered filamentous bacteriophage (phage) particles have unique potential in vaccine development due to their inherent immunogenicity, genetic plasticity, stability, cost-effectiveness for large-scale production, and proven safety profile in humans. Herein we report the development and initial evaluation of two targeted phage-based vaccination approaches against SARS-CoV-2: dual ligand peptide-targeted phage and adeno-associated virus/phage (AAVP) particles. For peptide-targeted phage, we performed structure-guided antigen design to select six solvent-exposed epitopes of the SARS-CoV-2 spike (S) protein. One of these epitopes displayed on the major capsid protein pVIII of phage induced a specific and sustained humoral response when injected in mice. These phage were further engineered to simultaneously display the peptide CAKSMGDIVC on the minor capsid protein pIII to enable their transport from the lung epithelium into the systemic circulation. Aerosolization of these “dual-display” phage into the lungs of mice generated a systemic and specific antibody response. In the second approach, targeted AAVP particles were engineered to deliver the entire S protein gene under the control of a constitutive CMV promoter. This induced tissue-specific transgene expression, stimulating a systemic S protein-specific antibody response in mice. With these proof-of-concept preclinical experiments, we show that both targeted phage- and AAVP-based particles serve as robust yet versatile platforms that can promptly yield COVID-19 vaccine prototypes for translational development. | - |
Descrição: dc.description | Rutgers Cancer Institute of New Jersey | - |
Descrição: dc.description | Division of Cancer Biology Department of Radiation Oncology Rutgers New Jersey Medical School | - |
Descrição: dc.description | Center for Theoretical Biological Physics Rice University | - |
Descrição: dc.description | Department of Physics Institute of Biosciences Humanities and Exact Sciences São Paulo State University | - |
Descrição: dc.description | Public Health Research Institute Rutgers New Jersey Medical School | - |
Descrição: dc.description | Department of Neurology Harvard Medical School | - |
Descrição: dc.description | Department of Surgery Rutgers Robert Wood Johnson Medical School | - |
Descrição: dc.description | Department of Physics and Center for Theoretical Biological Physics Northeastern University | - |
Descrição: dc.description | RCSB Protein Data Bank Institute for Quantitative Biomedicine, Rutgers State University of New Jersey | - |
Descrição: dc.description | Department of Chemistry and Chemical Biology Rutgers State University of New Jersey | - |
Descrição: dc.description | RCSB Protein Data Bank San Diego Supercomputer Center and Skaggs School of Pharmacy & Pharmaceutical Sciences University of California San Diego | - |
Descrição: dc.description | Department of Biosciences Rice University | - |
Descrição: dc.description | Department of Chemistry Rice University | - |
Descrição: dc.description | Department of Physics and Astronomy Rice University | - |
Descrição: dc.description | Division of Hematology/Oncology Department of Medicine Rutgers New Jersey Medical School | - |
Descrição: dc.description | Department of Physics Institute of Biosciences Humanities and Exact Sciences São Paulo State University | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Proceedings of the National Academy of Sciences of the United States of America | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | AAVP | - |
Palavras-chave: dc.subject | COVID-19 | - |
Palavras-chave: dc.subject | Gene delivery | - |
Palavras-chave: dc.subject | Phage display | - |
Palavras-chave: dc.subject | SARS-CoV-2 | - |
Título: dc.title | Design and proof of concept for targeted phage-based COVID-19 vaccination strategies with a streamlined cold-free supply chain | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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