Preparation of a magnetic molecularly imprinted polymer for non-invasive determination of cortisol

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorVilla, Javier E. L. [UNESP]-
Autor(es): dc.creatorKhan, Sabir [UNESP]-
Autor(es): dc.creatorNeres, Lucas C. S. [UNESP]-
Autor(es): dc.creatorSotomayor, Maria D. P. T. [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T22:10:24Z-
Data de disponibilização: dc.date.available2022-08-04T22:10:24Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10965-021-02659-y-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/221963-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/221963-
Descrição: dc.descriptionThis work presents the theoretical design, synthesis, characterization and application of a magnetic molecularly imprinted polymer (Mag-MIP) to quantify cortisol, the main stress biomarker, in urine samples. Density functional theory was used to predict a suitable solvent (acetonitrile) and a functional monomer (methacrylic acid) for MIP synthesis. Under these conditions, the Mag-MIP material was obtained by surface polymerization of modified Fe3O4 nanoparticles synthesized via the polyol method. The materials were characterized by scanning/transmission electron microscopy, infrared spectroscopy, vibrating sample magnetometry and thermogravimetric and Brunauer–Emmett–Teller surface analyses. Additionally, the Mag-MIP binding experiments showed rapid cortisol adsorption (completed in 20 min), Langmuir–Freundlich isotherm and a pseudo-first-order kinetic equation behaviour, and an excellent maximum binding capacity (200 µg g−1). The results were compared to those obtained for the magnetic non-imprinted polymer (Mag-NIP) and showed superior Mag-MIP performance. Selectivity experiments indicated that the Mag-MIP is capable of efficiently capturing cortisol in the presence of potential interfering substances. The Mag-MIP material was then applied to rapid quantitative cortisol extraction from urine samples prior to high-performance liquid chromatography with ultraviolet detection (limit of detection of 4 µg L−1 and recoveries ranging between 92 and 100%). Therefore, the proposed method might be readily implemented for routine non-invasive monitoring of human stress levels and other cortisol-related conditions.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionInstituto de Química Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionInstituto de Química Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionFAPESP: 2014/50945-4-
Descrição: dc.descriptionFAPESP: 2017/24196-2-
Descrição: dc.descriptionFAPESP: 2019/00677-7-
Idioma: dc.languageen-
Relação: dc.relationJournal of Polymer Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDFT-
Palavras-chave: dc.subjectHPLC–UV-
Palavras-chave: dc.subjectHydrocortisone-
Palavras-chave: dc.subjectMagnetite-
Palavras-chave: dc.subjectMIP-
Título: dc.titlePreparation of a magnetic molecularly imprinted polymer for non-invasive determination of cortisol-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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