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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Mikocheni Agriculture Research Institute (MARI) | - |
Autor(es): dc.contributor | University of Western Australia | - |
Autor(es): dc.contributor | National Agricultural Research Laboratories | - |
Autor(es): dc.contributor | Zhejiang University | - |
Autor(es): dc.contributor | Chinese Academy of Agricultural Sciences | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (UNESP) | - |
Autor(es): dc.contributor | DPIRD Diagnostic Laboratory Services | - |
Autor(es): dc.creator | Sseruwagi, Peter | - |
Autor(es): dc.creator | Wainaina, James | - |
Autor(es): dc.creator | Ndunguru, Joseph | - |
Autor(es): dc.creator | Tumuhimbise, Robooni | - |
Autor(es): dc.creator | Tairo, Fred | - |
Autor(es): dc.creator | Guo, Jian-Yang | - |
Autor(es): dc.creator | Vrielink, Alice | - |
Autor(es): dc.creator | Blythe, Amanda | - |
Autor(es): dc.creator | Kinene, Tonny | - |
Autor(es): dc.creator | De Marchi, Bruno [UNESP] | - |
Autor(es): dc.creator | Kehoe, Monica A. | - |
Autor(es): dc.creator | Tanz, Sandra | - |
Autor(es): dc.creator | Boykin, Laura M. | - |
Data de aceite: dc.date.accessioned | 2022-08-04T22:07:28Z | - |
Data de disponibilização: dc.date.available | 2022-08-04T22:07:28Z | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2017-01-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.12688/gatesopenres.12783.3 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/221143 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/221143 | - |
Descrição: dc.description | Background: Bemisia tabaci species (B. tabaci), or whiteflies, are the world’s most devastating insect pests. They cause billions of dollars (US) of damage each year, and are leaving farmers in the developing world food insecure. Currently, all publically available transcriptome data for B. tabaci are generated from pooled samples, which can lead to high heterozygosity and skewed representation of the genetic diversity. The ability to extract enough RNA from a single whitefly has remained elusive due to their small size and technological limitations. Methods: In this study, we optimised a single whitefly RNA extraction procedure, and sequenced the transcriptome of four individual adult Sub-Saharan Africa 1 (SSA1) B. tabaci. Transcriptome sequencing resulted in 39-42 million raw reads. De novo assembly of trimmed reads yielded between 65,000-162,000 Contigs across B. tabaci transcriptomes. Results: Bayesian phylogenetic analysis of mitochondrion cytochrome I oxidase (mtCOI) grouped the four whiteflies within the SSA1 clade. BLASTn searches on the four transcriptomes identified five endosymbionts; the primary endosymbiont Portiera aleyrodidarum and four secondary endosymbionts: Arsenophonus, Wolbachia, Rickettsia, and Cardinium spp. that were predominant across all four SSA1 B. tabaci samples with prevalence levels of between 54.1 to 75%. Amino acid alignments of the NusG gene of P. aleyrodidarum for the SSA1 B. tabaci transcriptomes of samples WF2 and WF2b revealed an eleven amino acid residue deletion that was absent in samples WF1 and WF2a. Comparison of the protein structure of the NusG protein from P. aleyrodidarum in SSA1 with known NusG structures showed the deletion resulted in a shorter D loop. Conclusions: The use of field-collected specimens means time and money will be saved in future studies using single whitefly transcriptomes in monitoring vector and viral interactions. Our method is applicable to any small organism where RNA quantity has limited transcriptome studies. | - |
Descrição: dc.description | Bill and Melinda Gates Foundation | - |
Descrição: dc.description | Department for International Development | - |
Descrição: dc.description | Mikocheni Agriculture Research Institute (MARI), P.O. Box 6226 | - |
Descrição: dc.description | School of Molecular Sciences and Australian Research Council Centre of Excellence in Plant Energy Biology University of Western Australia | - |
Descrição: dc.description | National Agricultural Research Laboratories, P.O. Box 7065, Kampala Kawanda-Senge Rd | - |
Descrição: dc.description | Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect Sciences Zhejiang University | - |
Descrição: dc.description | State Key Laboratory for the Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences | - |
Descrição: dc.description | Faculdade de Ciências Agronômicas Universidade Estadual Paulista | - |
Descrição: dc.description | Department of Primary Industries and Regional Development DPIRD Diagnostic Laboratory Services | - |
Descrição: dc.description | Faculdade de Ciências Agronômicas Universidade Estadual Paulista | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Gates Open Research | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Bacterial endosymbionts | - |
Palavras-chave: dc.subject | Cassava | - |
Palavras-chave: dc.subject | Next generation sequencing | - |
Palavras-chave: dc.subject | NusG | - |
Palavras-chave: dc.subject | Smallholder farmers | - |
Palavras-chave: dc.subject | Sub-Saharan Africa | - |
Título: dc.title | The first transcriptomes from field-collected individual whiteflies (Bemisia tabaci, hemiptera: Aleyrodidae): A case study of the endosymbiont composition | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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