In vitro schistosomicidal activity of the lignan ( )-6,60-dinitrohinokinin (dnhk) loaded into poly(lactic-co-glycolic acid) nanoparticles against schistosoma mansoni

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de Franca-
Autor(es): dc.contributorUniversidade Federal do Espírito Santo (UFES)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorLima, Thaís C.-
Autor(es): dc.creatorLucarini, Rodrigo-
Autor(es): dc.creatorLuz, Priscilla P.-
Autor(es): dc.creatorde Faria, Emerson H.-
Autor(es): dc.creatorMarçal, Liziane-
Autor(es): dc.creatorMagalhães, Lizandra G.-
Autor(es): dc.creatorBadoco, Fernanda R.-
Autor(es): dc.creatorEsperandim, Viviane R.-
Autor(es): dc.creatorMolina, Eduardo F.-
Autor(es): dc.creatorLaurentz, Rosangela S. [UNESP]-
Autor(es): dc.creatorLima, Regiane G. [UNESP]-
Autor(es): dc.creatorCunha, Wilson R.-
Autor(es): dc.creatorBastos, Jairo K.-
Autor(es): dc.creatorAndrade Silva, Marcio L.-
Data de aceite: dc.date.accessioned2022-08-04T22:07:20Z-
Data de disponibilização: dc.date.available2022-08-04T22:07:20Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2017-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/13880209.2017.1405996-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/221116-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/221116-
Descrição: dc.descriptionContext: ( )-6,60-Dinitrohinokinin (DNHK) display remarkable antiparasitic activity and was, therefore, incorporated into a nanoparticle formulation. Objective: Incorporation of DNHK in poly lactic-co-glycolic acid (PLGA) nanoparticles aiming to improve its biological activities. Materials and methods: Synthesis, characterization and incorporation of DNHK into glycolic acid (PLGA) nanoparticles by nanoprecipitation method. The nanoparticles were characterized by ultraviolet-visible spectroscopy, X-ray diffraction, field emission electron microscopic scanning mansoni (FESEM), and dynamic light scattering (DLS). For the in vitro test with Schistosoma mansoni, the DNHK-loaded PLGA was diluted into the medium, and added at concentrations 10–200 mM to the culture medium containing one adult worm pair. The parasites were kept for 120 h and monitored every 24 h to evaluate their general condition, including: pairing, alterations in motor activity and mortality. Results: The loaded PLGA nanoparticles gave an encapsulation efficiency of 42.2% and showed spherical characteristics in monodisperse polymeric matrix. The adult worm pairs were separated after 120 h of incubation for concentrations higher than 50 mM of DNHK-loaded PLGA. The groups incubated with 150 and 200 mM of DNHK-loaded PLGA for 24 and 120 h killed 100% of adult worms, afforded LC50 values of 137.0 ± 2.12 mM and 79.01 ± 1.90 mM, respectively, which was similar to the effect displayed by 10 mM of praziquantel. Discussion and conclusions: The incorporation of DNHK-loaded showed schistosomicidal activity and allowed its sustained release. The loaded PLGA system can be administered intravenously, as well as it may be internalized by endocytosis by the target organisms.-
Descrição: dc.descriptionLaboratório de Pesquisa em Microbiologia Aplicada Núcleo de Ciências Exatas e Tecnológicas Universidade de Franca-
Descrição: dc.descriptionDepartamento de Química–CCE Universidade Federal do Espírito Santo (UFES)-
Descrição: dc.descriptionFaculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo-
Descrição: dc.descriptionFaculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista-
Formato: dc.format2270-2276-
Idioma: dc.languageen-
Relação: dc.relationPharmaceutical Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject6, 60-dinitrohinokinin-
Palavras-chave: dc.subjectBiological activity-
Palavras-chave: dc.subjectNanoparticulate formulation-
Palavras-chave: dc.subjectPLGA-
Palavras-chave: dc.subjectSchistosomicidal-
Título: dc.titleIn vitro schistosomicidal activity of the lignan ( )-6,60-dinitrohinokinin (dnhk) loaded into poly(lactic-co-glycolic acid) nanoparticles against schistosoma mansoni-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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