Quantitative peptidomics using reductive methylation of amines

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorAlbert Einstein College of Medicine-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorDasgupta, Sayani-
Autor(es): dc.creatorCastro, Leandro M. [UNESP]-
Autor(es): dc.creatorTashima, Alexandre K.-
Autor(es): dc.creatorFricker, Lloyd-
Data de aceite: dc.date.accessioned2022-08-04T22:07:00Z-
Data de disponibilização: dc.date.available2022-08-04T22:07:00Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2018-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/978-1-4939-7537-2_10-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/221026-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/221026-
Descrição: dc.descriptionA number of different approaches have been used for quantitative peptidomics. In this protocol we describe the method in which peptides are reacted with formaldehyde and sodium cyanoborohydride, which converts primary and secondary amines into tertiary amines. By using different combinations of regular reagents, deuterated reagents (2H), and reagents containing deuterium and 13C, it is possible to produce five isotopically distinct forms of the methylated peptides which can be quantified by mass spectrometry. Peptides with free N-termini that are primary amines incorporate two methyl groups using this procedure, which differ by 2 Da for each of the five isotopic combinations. Peptides that contain unmodified lysine residues incorporate additional pairs of methyl groups, leading to larger mass differences between isotopic forms. The reagents are commercially available, relatively inexpensive, and chemically stable.-
Descrição: dc.descriptionDepartment of Molecular Pharmacology Albert Einstein College of Medicine-
Descrição: dc.descriptionBioscience Institute São Paulo State University-
Descrição: dc.descriptionDepartment of Biochemistry Escola Paulista de Medicina Federal University of São Paulo-
Descrição: dc.descriptionDepartment of Neuroscience Albert Einstein College of Medicine-
Descrição: dc.descriptionBioscience Institute São Paulo State University-
Formato: dc.format161-174-
Idioma: dc.languageen-
Relação: dc.relationMethods in Molecular Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCyanoborohydride-
Palavras-chave: dc.subjectFormaldehyde-
Palavras-chave: dc.subjectIsotopic labels-
Título: dc.titleQuantitative peptidomics using reductive methylation of amines-
Aparece nas coleções:Repositório Institucional - Unesp

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