A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells

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Autor(es): dc.contributorAgriculture and Agri-Food Canada-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Alberta-
Autor(es): dc.contributorStellenbosch University-
Autor(es): dc.creatorVahmani, Payam-
Autor(es): dc.creatorMeadus, William J.-
Autor(es): dc.creatorda Silva, Maria L.P. [UNESP]-
Autor(es): dc.creatorMitchell, Alec D.-
Autor(es): dc.creatorMapiye, Cletos-
Autor(es): dc.creatorDuff, Pascale-
Autor(es): dc.creatorRolland, David C.-
Autor(es): dc.creatorDugan, Michael E.R.-
Data de aceite: dc.date.accessioned2022-08-04T22:05:35Z-
Data de disponibilização: dc.date.available2022-08-04T22:05:35Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2016-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bbrep.2016.05.018-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/220632-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/220632-
Descrição: dc.descriptionBeef fat is a natural source of trans (t) fatty acids, and is typically enriched with either t10-18:1 or t11-18:1. Little is known about the bioactivity of individual t-18:1 isomers, and the present study compared the effects of t9-18:1, cis (c)9-18:1 and trans (t)-18:1 fractions isolated from beef fat enriched with either t10-18:1 (HT10) or t11-18:1 (HT11). All 18:1 isomers resulted in reduced human liver (HepG2) cell viability relative to control. Both c9-18:1 and HT11were the least toxic, t9-18:1had dose response increased toxicity, and HT10 had the greatest toxicity (P<0.05). Incorporation of t18:1 isomers was 1.8-2.5 fold greater in triacylglycerol (TG) than phospholipids (PL), whereas δ9 desaturation products were selectively incorporated into PL. Culturing HepG2 cells with t9-18:1 and HT10 increased (P<0.05) the δ9 desaturation index (c9-16:1/16:0) compared to other fatty acid treatments. HT10 and t9-18:1 also increased expression of lipogenic genes (FAS, SCD1, HMGCR and SREBP2) compared to control (P<0.05), whereas c9-18:1 and HT11 did not affect the expression of these genes. Our results suggest effects of HT11 and c9-18:1 were similar to BSA control, whereas HT10 and t-9 18:1 (i.e. the predominant trans fatty acid isomer found in partially hydrogenated vegetable oils) were more cytotoxic and led to greater expression of lipogenic genes.-
Descrição: dc.descriptionLacombe Research and Development Centre Agriculture and Agri-Food Canada-
Descrição: dc.descriptionFaculty of Veterinary and Agricultural Sciences São Paulo State University-
Descrição: dc.descriptionAculty of Science University of Alberta-
Descrição: dc.descriptionDepartment of Animal Sciences Faculty of AgriSciences Stellenbosch University, P. Bag X1-
Descrição: dc.descriptionFaculty of Veterinary and Agricultural Sciences São Paulo State University-
Formato: dc.format84-90-
Idioma: dc.languageen-
Relação: dc.relationBiochemistry and Biophysics Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBeef-
Palavras-chave: dc.subjectCell culture-
Palavras-chave: dc.subjectCytotoxicity-
Palavras-chave: dc.subjectFatty acid metabolism-
Palavras-chave: dc.subjectLiver-
Palavras-chave: dc.subjectTrans fatty acids-
Título: dc.titleA trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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