Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Agriculture and Agri-Food Canada | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (UNESP) | - |
Autor(es): dc.contributor | University of Alberta | - |
Autor(es): dc.contributor | Stellenbosch University | - |
Autor(es): dc.creator | Vahmani, Payam | - |
Autor(es): dc.creator | Meadus, William J. | - |
Autor(es): dc.creator | da Silva, Maria L.P. [UNESP] | - |
Autor(es): dc.creator | Mitchell, Alec D. | - |
Autor(es): dc.creator | Mapiye, Cletos | - |
Autor(es): dc.creator | Duff, Pascale | - |
Autor(es): dc.creator | Rolland, David C. | - |
Autor(es): dc.creator | Dugan, Michael E.R. | - |
Data de aceite: dc.date.accessioned | 2022-08-04T22:05:35Z | - |
Data de disponibilização: dc.date.available | 2022-08-04T22:05:35Z | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2016-09-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1016/j.bbrep.2016.05.018 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/220632 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/220632 | - |
Descrição: dc.description | Beef fat is a natural source of trans (t) fatty acids, and is typically enriched with either t10-18:1 or t11-18:1. Little is known about the bioactivity of individual t-18:1 isomers, and the present study compared the effects of t9-18:1, cis (c)9-18:1 and trans (t)-18:1 fractions isolated from beef fat enriched with either t10-18:1 (HT10) or t11-18:1 (HT11). All 18:1 isomers resulted in reduced human liver (HepG2) cell viability relative to control. Both c9-18:1 and HT11were the least toxic, t9-18:1had dose response increased toxicity, and HT10 had the greatest toxicity (P<0.05). Incorporation of t18:1 isomers was 1.8-2.5 fold greater in triacylglycerol (TG) than phospholipids (PL), whereas δ9 desaturation products were selectively incorporated into PL. Culturing HepG2 cells with t9-18:1 and HT10 increased (P<0.05) the δ9 desaturation index (c9-16:1/16:0) compared to other fatty acid treatments. HT10 and t9-18:1 also increased expression of lipogenic genes (FAS, SCD1, HMGCR and SREBP2) compared to control (P<0.05), whereas c9-18:1 and HT11 did not affect the expression of these genes. Our results suggest effects of HT11 and c9-18:1 were similar to BSA control, whereas HT10 and t-9 18:1 (i.e. the predominant trans fatty acid isomer found in partially hydrogenated vegetable oils) were more cytotoxic and led to greater expression of lipogenic genes. | - |
Descrição: dc.description | Lacombe Research and Development Centre Agriculture and Agri-Food Canada | - |
Descrição: dc.description | Faculty of Veterinary and Agricultural Sciences São Paulo State University | - |
Descrição: dc.description | Aculty of Science University of Alberta | - |
Descrição: dc.description | Department of Animal Sciences Faculty of AgriSciences Stellenbosch University, P. Bag X1 | - |
Descrição: dc.description | Faculty of Veterinary and Agricultural Sciences São Paulo State University | - |
Formato: dc.format | 84-90 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Biochemistry and Biophysics Reports | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Beef | - |
Palavras-chave: dc.subject | Cell culture | - |
Palavras-chave: dc.subject | Cytotoxicity | - |
Palavras-chave: dc.subject | Fatty acid metabolism | - |
Palavras-chave: dc.subject | Liver | - |
Palavras-chave: dc.subject | Trans fatty acids | - |
Título: dc.title | A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: