Analysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorSchool of Pharmacy, University of Rio Grande Do sul - UFRGS-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFaculty of Health Sciences, Fernando Pessoa University (FCS-UFP)-
Autor(es): dc.contributorInstitute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (CGB-UTAD/IBB)-
Autor(es): dc.creatorSeverino, Patrícia-
Autor(es): dc.creatorSilva, Heloisa [UNESP]-
Autor(es): dc.creatorSouto, Eliana B.-
Autor(es): dc.creatorSantana, Maria Helena A.-
Autor(es): dc.creatorDalla Costa, Teresa Cristina T.-
Data de aceite: dc.date.accessioned2022-08-04T22:04:33Z-
Data de disponibilização: dc.date.available2022-08-04T22:04:33Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2012-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jpha.2011.10.006-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/220332-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/220332-
Descrição: dc.descriptionDidanosine is an effective antiviral drug in untreated and antiretroviral therapy-experienced patients with Human Immunodeficiency Virus (HIV). An automated system using on-line solid extraction and High Performance Liquid Chromatography (HPLC) with ultraviolet (UV) detection was developed and validated for pharmacokinetic analysis of didanosine in dog plasma. Modifications were introduced on a previous methodology for simultaneous analysis of antiretroviral drugs in human plasma. Extraction was carried out on C18 cartridges, with high extraction yield as stationary phase, whereas mobile phase consisted of a mixture of 0.02 M potassium phosphate buffer, acetonitrile (KH2PO4: acetonitrile: 96:4, v/v) and 0.5% (w/v) of heptane sulphonic acid. The pH was adjusted to 6.5 with triethylamine. All samples and standard solutions were chromatographed at 28 °C. For an isocratic run, the flux was 1.0 mL/min, detection was at 250 nm and injected volume was 20 μL. The method was selective and linear for concentrations between 50 and 5000 ng/mL. Drug stability data ranged from 96% to 98%, and limit of quantification was 25 ng/mL. Extraction yield was up to 95%. Drug stability in dog plasma was kept frozen at -20 °C for one month after three freezethaw cycles, and for 24 h after processing in the auto sampler. Assay was successfully applied to measure didanosine concentrations in plasma dogs. © 2012 Xian Jiaotong University. Productionand hosting by Elsevier B.V. All rights reserved.-
Descrição: dc.descriptionBiotechnological Processes Development Laboratory, School of Chemical Engineering, State University of Campinas UNICAMP, Campinas, SP-
Descrição: dc.descriptionSchool of Pharmacy, University of Rio Grande Do sul - UFRGS, Porto Alegre, Rio Grande do Sul 90610-000-
Descrição: dc.descriptionSchool of Agrarian Science and Veterinary, São Paulo State University - UNESP, Jaboticabal, São Paulo 14.884-900-
Descrição: dc.descriptionFaculty of Health Sciences, Fernando Pessoa University (FCS-UFP), Rua Carlos da Maia, 296, P-4200-150 Porto-
Descrição: dc.descriptionInstitute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (CGB-UTAD/IBB), P.O. Box 1013, P-5001-801, Vila Real-
Descrição: dc.descriptionSchool of Agrarian Science and Veterinary, São Paulo State University - UNESP, Jaboticabal, São Paulo 14.884-900-
Formato: dc.format29-34-
Idioma: dc.languageen-
Relação: dc.relationJournal of Pharmaceutical Analysis-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDidanosine-
Palavras-chave: dc.subjectHPLC-
Palavras-chave: dc.subjectMale dogs-
Palavras-chave: dc.subjectOn-line solid phase extraction-
Título: dc.titleAnalysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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