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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual de Campinas (UNICAMP) | - |
Autor(es): dc.contributor | School of Pharmacy, University of Rio Grande Do sul - UFRGS | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (UNESP) | - |
Autor(es): dc.contributor | Faculty of Health Sciences, Fernando Pessoa University (FCS-UFP) | - |
Autor(es): dc.contributor | Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (CGB-UTAD/IBB) | - |
Autor(es): dc.creator | Severino, Patrícia | - |
Autor(es): dc.creator | Silva, Heloisa [UNESP] | - |
Autor(es): dc.creator | Souto, Eliana B. | - |
Autor(es): dc.creator | Santana, Maria Helena A. | - |
Autor(es): dc.creator | Dalla Costa, Teresa Cristina T. | - |
Data de aceite: dc.date.accessioned | 2022-08-04T22:04:33Z | - |
Data de disponibilização: dc.date.available | 2022-08-04T22:04:33Z | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2022-04-28 | - |
Data de envio: dc.date.issued | 2012-01-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1016/j.jpha.2011.10.006 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/220332 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/220332 | - |
Descrição: dc.description | Didanosine is an effective antiviral drug in untreated and antiretroviral therapy-experienced patients with Human Immunodeficiency Virus (HIV). An automated system using on-line solid extraction and High Performance Liquid Chromatography (HPLC) with ultraviolet (UV) detection was developed and validated for pharmacokinetic analysis of didanosine in dog plasma. Modifications were introduced on a previous methodology for simultaneous analysis of antiretroviral drugs in human plasma. Extraction was carried out on C18 cartridges, with high extraction yield as stationary phase, whereas mobile phase consisted of a mixture of 0.02 M potassium phosphate buffer, acetonitrile (KH2PO4: acetonitrile: 96:4, v/v) and 0.5% (w/v) of heptane sulphonic acid. The pH was adjusted to 6.5 with triethylamine. All samples and standard solutions were chromatographed at 28 °C. For an isocratic run, the flux was 1.0 mL/min, detection was at 250 nm and injected volume was 20 μL. The method was selective and linear for concentrations between 50 and 5000 ng/mL. Drug stability data ranged from 96% to 98%, and limit of quantification was 25 ng/mL. Extraction yield was up to 95%. Drug stability in dog plasma was kept frozen at -20 °C for one month after three freezethaw cycles, and for 24 h after processing in the auto sampler. Assay was successfully applied to measure didanosine concentrations in plasma dogs. © 2012 Xian Jiaotong University. Productionand hosting by Elsevier B.V. All rights reserved. | - |
Descrição: dc.description | Biotechnological Processes Development Laboratory, School of Chemical Engineering, State University of Campinas UNICAMP, Campinas, SP | - |
Descrição: dc.description | School of Pharmacy, University of Rio Grande Do sul - UFRGS, Porto Alegre, Rio Grande do Sul 90610-000 | - |
Descrição: dc.description | School of Agrarian Science and Veterinary, São Paulo State University - UNESP, Jaboticabal, São Paulo 14.884-900 | - |
Descrição: dc.description | Faculty of Health Sciences, Fernando Pessoa University (FCS-UFP), Rua Carlos da Maia, 296, P-4200-150 Porto | - |
Descrição: dc.description | Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (CGB-UTAD/IBB), P.O. Box 1013, P-5001-801, Vila Real | - |
Descrição: dc.description | School of Agrarian Science and Veterinary, São Paulo State University - UNESP, Jaboticabal, São Paulo 14.884-900 | - |
Formato: dc.format | 29-34 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Journal of Pharmaceutical Analysis | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Didanosine | - |
Palavras-chave: dc.subject | HPLC | - |
Palavras-chave: dc.subject | Male dogs | - |
Palavras-chave: dc.subject | On-line solid phase extraction | - |
Título: dc.title | Analysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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