Vitrification of bovine oocytes at different meiotic stages using the Cryotop method: Assessment of morphological, molecular and functional patterns

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Brasilia-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorAnimal Reproduction Inc.-
Autor(es): dc.creatorSprícigo, J. F.W.-
Autor(es): dc.creatorMorais, K.-
Autor(es): dc.creatorFerreira, A. R.-
Autor(es): dc.creatorMachado, G. M.-
Autor(es): dc.creatorGomes, A. C.M.-
Autor(es): dc.creatorRumpf, R.-
Autor(es): dc.creatorFranco, M. M.-
Autor(es): dc.creatorDode, M. A.N.-
Data de aceite: dc.date.accessioned2022-08-04T22:04:16Z-
Data de disponibilização: dc.date.available2022-08-04T22:04:16Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2014-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.cryobiol.2014.07.015-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/220239-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/220239-
Descrição: dc.descriptionThis study aimed to investigate the functional, morphological and molecular patterns of bovine oocytes vitrified at different times during in vitro maturation (IVM). Four groups of oocytes were used: non-vitrified control oocytes (CG), oocytes vitrified at 0. h (V0), oocytes vitrified after 8. h of IVM (V8) and oocytes vitrified after 22. h of IVM (V22). After vitrification, the oocytes were warmed and then returned to the incubator to complete a total of 24. h of IVM. To evaluate the effect of vitrification, the nuclear maturation and fertilization rates were assessed by lacmoid staining and ultrastructural electron microscopy. The cleavage and blastocyst rates were evaluated at D2, D7 and D8. The expression levels of CASP3, TP53, HDAC2, SUV39H1 and DNMT1 were investigated by RT-qPCR. The nuclear maturation, oocyte fertilization, cleavage and blastocyst rates were higher (P<. 0.05) in the CG group (80%; 81.3%; 88.5%; and 35.8%) than in the V0 (44%; 44.6%; 22.7%; and 2.6%), V8 (50%; 63%; 21.5%; and 2.2%) and V22 (55.5%; 66.9%; 24.1%; and 4.6%) groups. Ultrastructural analysis revealed significant damage within the cytoplasm of all vitrified groups, but more severe degeneration was observed in the V22 group. The gene expression profiles were not affected by vitrification (P>0.05). In conclusion, cytoplasm degeneration seems to be the most severe form of damage caused by vitrification. The use of the Cryotop method for vitrification severely reduces bovine oocyte viability regardless of whether it is performed at GV, GVBD or MII stage.-
Descrição: dc.descriptionSchool of Agriculture and Veterinary Medicine University of Brasilia-
Descrição: dc.descriptionEmbrapa Genetic Resources and Biotechnology Laboratory of Animal Reproduction-
Descrição: dc.descriptionSchool of Veterinary Medicine University of São Paulo State-
Descrição: dc.descriptionGeneal Animal Reproduction Inc.-
Formato: dc.format256-265-
Idioma: dc.languageen-
Relação: dc.relationCryobiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBovine-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectUltrastructure-
Palavras-chave: dc.subjectVitrification-
Título: dc.titleVitrification of bovine oocytes at different meiotic stages using the Cryotop method: Assessment of morphological, molecular and functional patterns-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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