Mycelial biomass and biochemical properties of proteases produced by Lentinus citrinus DPUA 1535 (Higher Basidiomycetes) in submerged cultivation

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Amazonas-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorKirsch, Larissa de Souza-
Autor(es): dc.creatorEbinuma, Valéria de Carvalho Santos [UNESP]-
Autor(es): dc.creatorTeixeira, Maria Francisca Simas-
Data de aceite: dc.date.accessioned2022-08-04T22:03:26Z-
Data de disponibilização: dc.date.available2022-08-04T22:03:26Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2013-10-25-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1615/IntJMedMushr.v15.i5.80-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/219957-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/219957-
Descrição: dc.descriptionThe cultivation of Lentinus citrinus for mycelial biomass and protease production under different carbon and nitrogen sources was studied in submerged cultivation. The nutritional source concentration for protease production was evaluated using a full factorial design. For mycelial biomass maltose (4.94 mg/mL) and beef extract (5.45 mg/mL), carbon and nitrogen sources presented the best results, respectively. The maximum protease activity was 73.33 U/mL with fructose (30.0 g/L) and beef extract (10.0 g/L). Proteases showed maximum activity at 40°C and pH 7.0, which exhibited high stability at experimental conditions. The final part of this work was devoted to estimating the main thermodynamic parameters of the irreversible enzyme inactivation (ΔH* = 17.86 kJ/mol, ΔG* =102.09 kJ/mol, ΔS* = -260.76 J/mol×K) through residual activity tests carried out at 25-70°C, by making use of Arrhenius and Eyring plots. © 2013 Begell House, Inc.-
Descrição: dc.descriptionCulture Collection DPUA Federal University of Amazonas, Manaus-
Descrição: dc.descriptionDepartment of Bioprocess and Biotechnology School of Pharmaceutical Sciences UNESP-Univ. Estadual Paulista, Araraquara-
Descrição: dc.descriptionDepartment of Bioprocess and Biotechnology School of Pharmaceutical Sciences UNESP-Univ. Estadual Paulista, Araraquara-
Formato: dc.format505-515-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Medicinal Mushrooms-
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Palavras-chave: dc.subjectLentinus citrinus-
Palavras-chave: dc.subjectMedicinal mushrooms-
Palavras-chave: dc.subjectMycelial biomass-
Palavras-chave: dc.subjectNitrogen and carbon sources-
Palavras-chave: dc.subjectProtease activity-
Título: dc.titleMycelial biomass and biochemical properties of proteases produced by Lentinus citrinus DPUA 1535 (Higher Basidiomycetes) in submerged cultivation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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