Molecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.creatorPaixão, Douglas Antonio Alvaredo [UNESP]-
Autor(es): dc.creatorDimitrov, Mauricio Rocha-
Autor(es): dc.creatorPereira, Rodrigo Matheus-
Autor(es): dc.creatorAccorsini, Fábio Raphael [UNESP]-
Autor(es): dc.creatorVidotti, Maria Benincasa [UNESP]-
Autor(es): dc.creatorde Macedo Lemos, Eliana Gertrudes [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T22:02:10Z-
Data de disponibilização: dc.date.available2022-08-04T22:02:10Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2010-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/s0100-06832010000300019-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/219587-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/219587-
Descrição: dc.descriptionDiesel oil is a compound derived from petroleum, consisting primarily of hydrocarbons. Poor conditions in transportation and storage of this product can contribute significantly to accidental spills causing serious ecological problems in soil and water and affecting the diversity of the microbial environment. The cloning and sequencing of the 16S rRNA gene is one of the molecular techniques that allows estimation and comparison of the microbial diversity in different environmental samples. The aim of this work was to estimate the diversity of microorganisms from the Bacteria domain in a consortium specialized in diesel oil degradation through partial sequencing of the 16S rRNA gene. After the extraction of DNA metagenomics, the material was amplified by PCR reaction using specific oligonucleotide primers for the 16S rRNA gene. The PCR products were cloned into a pGEM-T-Easy vector (Promega), and Escherichia coli was used as the host cell for recombinant DNAs. The partial clone sequencing was obtained using universal oligonucleotide primers from the vector. The genetic library obtained generated 431 clones. All the sequenced clones presented similarity to phylum Proteobacteria, with Gammaproteobacteria the most present group (49.8 % of the clones), followed by Alphaproteobacteira (44.8 %) and Betaproteobacteria (5.4 %). The Pseudomonas genus was the most abundant in the metagenomic library, followed by the Parvibaculum and the Sphingobium genus, respectively. After partial sequencing of the 16S rRNA, the diversity of the bacterial consortium was estimated using DOTUR software. When comparing these sequences to the database from the National Center for Biotechnology Information (NCBI), a strong correlation was found between the data generated by the software used and the data deposited in NCBI.-
Descrição: dc.descriptionFCAV UNESP - São Paulo State University, Rod. Paulo Donato Castellane s/n, CEP 14884-900 Jaboticabal, São Paulo-
Descrição: dc.descriptionUniversity of São Paulo (USP)-
Descrição: dc.descriptionEmbrapa Soybean, Rod Carlos João Strass - Distrito de Warta, CEP 86001-970 Londrina- Paraná-
Descrição: dc.descriptionFCAV UNESP - São Paulo State University-
Descrição: dc.descriptionFCAV UNESP - São Paulo State University, Rod. Paulo Donato Castellane s/n, CEP 14884-900 Jaboticabal, São Paulo-
Descrição: dc.descriptionFCAV UNESP - São Paulo State University-
Formato: dc.format773-781-
Idioma: dc.languagept_BR-
Relação: dc.relationRevista Brasileira de Ciencia do Solo-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject16s rRNA-
Palavras-chave: dc.subjectBiosurfactants-
Palavras-chave: dc.subjectCloning-
Palavras-chave: dc.subjectMetagenomics-
Palavras-chave: dc.subjectPCR-
Palavras-chave: dc.subjectSoil-
Título: dc.titleMolecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation-
Título: dc.titleAnálise molecular da diversidade bacteriana de um consórcio degradador de óleo diesel-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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