Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorMedical School of the University of Porto-
Autor(es): dc.contributorHospital São João-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorSchmitt, Fernando Carlos [UNESP]-
Autor(es): dc.creatorBento, Maria José [UNESP]-
Autor(es): dc.creatorAmendoeira, Isabel [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T22:00:58Z-
Data de disponibilização: dc.date.available2022-08-04T22:00:58Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued1995-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/dc.2840130417-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/219176-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/219176-
Descrição: dc.descriptionWe describe a method of immunocytochemically assessing estrogen receptor (ER) status on alcohol‐fixed smears obtained by fine‐needle aspiration (FNA) from breast cancer patients, using a commercially available monoclonal antibody (1D5) with microwave oven processing. A series of 31 cases of aspirates from breast cancer were analysed and the results were compared with assessment by ER immunocytochemical assay using the same procedure on formalin‐fixed tissue and with assessment by ER‐ICA assay on frozen sections. The results were scored semiquantitatively using a five grade scoring system. Of the 31 cases examined, 21 were positive at least by two methods and 10 were negative for all three determinations. The results obtained in the ER immunocytochemical assay on aspirates and paraffin‐sections using the antibody 1D5 and those obtained on frozen sections using the antibody H222 were closely similar. In only one case was it not possible to interpret the reaction in the cytological specimen because there was a strong background in the smear. In general, we obtained more intense positivity with the antibody 1D5 in aspirates and formalin‐fixed material than with the antibody H222 in frozen sections. The scoring results of the three methods were almost identical. We conclude that the application of ER method on alcohol‐fixed smears will eliminate the need for using a special fixation procedure and will provide several advantages, such as: improvement in morphological concomitant analysis, utilization whenever malignancy is found without necessity to re‐aspirate the patient, and adequacy of archival material. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company-
Descrição: dc.descriptionInstitute of Molecular Pathology and Immunology—IPATIMUP Medical School of the University of Porto-
Descrição: dc.descriptionCytopathology Unit Department of Pathology Hospital São João, Porto-
Descrição: dc.descriptionFNA Ambulatory Service Botucatu School of Medicine UNESP, Botucatu-
Descrição: dc.descriptionFNA Ambulatory Service Botucatu School of Medicine UNESP, Botucatu-
Formato: dc.format347-351-
Idioma: dc.languageen-
Relação: dc.relationDiagnostic Cytopathology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBreast cancer-
Palavras-chave: dc.subjectEstrogen receptor-
Palavras-chave: dc.subjectFNA-
Título: dc.titleEstimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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