The pho-2A mutant of Neurospora crassa which is deficient in Pi-repressible alkaline phosphatase (EC 3.1.3.1) is also defective in Pi-repressible acid phosphatase (EC 3.1.3.2).

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorHan, S. W. [UNESP]-
Autor(es): dc.creatorMaccheroni, W. [UNESP]-
Autor(es): dc.creatorRossi, A. [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T22:00:54Z-
Data de disponibilização: dc.date.available2022-08-04T22:00:54Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued1992-01-01-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/219155-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/219155-
Descrição: dc.description1. The mycelial Pi-repressible acid phosphatase presented p-nitrophenylphosphatase activity with negative cooperativity and Michaelian behavior when synthesized by the wild-type and pho-2A mutant strains of Neurospora crassa, respectively. 2. The major acid phosphatase present in cell extracts of the pho-2A mutant of N. crassa grown in low Pi medium is more thermolabile (t1/2 = 4 min at 54 degrees C, pH 5.4) than that of the wild strain (stable for at least 80 min at 54 degrees C, pH 5.4). 3. The pho-2A mutant of N. crassa secreted a more thermolabile acid phosphatase (t1/2 = 30 min at 50 degrees C, pH 5.4) than the wild strain (t1/2 of at least 80 min at 50 degrees C, pH 5.4). 4. The pho-2A mutant of N. crassa synthesized a more thermolabile acid phosphatase (t1/2 = 37 min at 54 degrees C, pH 5.4) than the wild strain in high Pi medium (t1/2 = 14 min at 54 degrees C, pH 5.4). 5. The pleiotropic nature of the pho-2 locus and its possible involvement in the mechanism of phosphatase secretion by N. crassa are proposed.-
Descrição: dc.descriptionDepartamento de Bioquímica e Microbiologia Universidade Estadual Paulista-
Descrição: dc.descriptionDepartamento de Bioquímica e Microbiologia Universidade Estadual Paulista-
Formato: dc.format441-447-
Idioma: dc.languageen-
Relação: dc.relationBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]-
???dc.source???: dc.sourceScopus-
Título: dc.titleThe pho-2A mutant of Neurospora crassa which is deficient in Pi-repressible alkaline phosphatase (EC 3.1.3.1) is also defective in Pi-repressible acid phosphatase (EC 3.1.3.2).-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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