Influence of Vitrification Device, Warming Protocol, and Subsequent In Vitro Culture on Structural Integrity of Testicular Fragments from Adult Domestic Cats

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Autor(es): dc.contributorUniv Brasil-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorInst Fed Minas Gerais-
Autor(es): dc.contributorNatl Zool Pk-
Autor(es): dc.creatorMacente, Beatrice Ingrid-
Autor(es): dc.creatorFonseca-Alves, Carlos Eduardo [UNESP]-
Autor(es): dc.creatorMagalhaes, Georgia Mode-
Autor(es): dc.creatorTavares, Mariana Riboli [UNESP]-
Autor(es): dc.creatorMansano, Cleber Fernando Menegasso-
Autor(es): dc.creatorMouttham, Lara-
Autor(es): dc.creatorApparicio, Maricy [UNESP]-
Autor(es): dc.creatorToniollo, Gilson Helio [UNESP]-
Autor(es): dc.creatorComizzoli, Pierre-
Data de aceite: dc.date.accessioned2022-08-04T21:59:49Z-
Data de disponibilização: dc.date.available2022-08-04T21:59:49Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-01-10-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1089/bio.2021.0092-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/218954-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/218954-
Descrição: dc.descriptionThe objective of the study was to evaluate the integrity of cat testicular tissues after vitrification with different devices followed by different warming conditions. The influence of vitro culture for 24 hours after warming also was examined. Testicular tissues from adult domestic cats were dissected in small fragments that were vitrified using Cryotop(R) or threaded on fine needles, warmed (directly at 37 degrees C or with a preliminary 10 seconds exposure to 50 degrees C), and/or cultured in vitro for an additional 24 hours. For each treatment group, tissues were assessed based on histology, apoptosis, and sperm DNA integrity. Results showed that fragments of testicular tissues were efficiently cryopreserved (maintaining the quality of all cell types) with vitrification with Cryotop followed by direct warming at 37 degrees C, and additional culture of 24 hours at 38.5 degrees C. These encouraging results are paving the road to optimize preservation protocols and use them for systematic banking of tissues from genetically valuable felids.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniv Brasil, Dept Med Vet, Estr Pojetada F1, BR-15600000 Fernandopolis, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista UNESP, Fac Med Vet & Zootecnia FMVZ, Botucatu, SP, Brazil-
Descrição: dc.descriptionInst Fed Minas Gerais, Muzambinho, MG, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista UNESP, Fac Ciencias Agr & Vet FCAV, Jaboticabal, SP, Brazil-
Descrição: dc.descriptionNatl Zool Pk, Smithsonian Conservat Biol Inst, Washington, DC USA-
Descrição: dc.descriptionUniv Estadual Paulista UNESP, Fac Med Vet & Zootecnia FMVZ, Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista UNESP, Fac Ciencias Agr & Vet FCAV, Jaboticabal, SP, Brazil-
Descrição: dc.descriptionCAPES: 001 (PDSE-CAPES-88881.1323 32/2016-01)-
Formato: dc.format9-
Idioma: dc.languageen-
Publicador: dc.publisherMary Ann Liebert, Inc-
Relação: dc.relationBiopreservation And Biobanking-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjecttesticular tissue-
Palavras-chave: dc.subjectdomestic cat-
Palavras-chave: dc.subjectvitrification-
Palavras-chave: dc.subjectcryodevice-
Palavras-chave: dc.subjectstructural integrity-
Título: dc.titleInfluence of Vitrification Device, Warming Protocol, and Subsequent In Vitro Culture on Structural Integrity of Testicular Fragments from Adult Domestic Cats-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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