Double-grafted chitosans as siRNA nanocarriers: effects of diisopropylethylamine substitution and labile-PEG coating

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniv Montreal-
Autor(es): dc.creatorMartinez Junior, Andre Miguel [UNESP]-
Autor(es): dc.creatorFelix Viegas de Souza, Ricchard Hallan [UNESP]-
Autor(es): dc.creatorPetronio, Maicon Segalla [UNESP]-
Autor(es): dc.creatorMartins, Grazieli Olinda [UNESP]-
Autor(es): dc.creatorFernandes, Julio Cesar-
Autor(es): dc.creatorBenderdour, Mohamed-
Autor(es): dc.creatorOliveira de Tiera, Vera Aparecida [UNESP]-
Autor(es): dc.creatorTiera, Marcio Jose [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T21:59:27Z-
Data de disponibilização: dc.date.available2022-08-04T21:59:27Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-03-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s40097-022-00487-0-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/218855-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/218855-
Descrição: dc.descriptionThe preparation of safe and efficient siRNA carriers remains a challenge that has limited the therapeutic applications of siRNA. In this study, the design of a new small interfering RNA (siRNA) carrier based on diisopropylaminoethyl-chitosan was devised for application in non-viral gene therapy. Polycations having varied proportions (11-32%) of diisopropylethylamine groups (DIPEA) and grafted with polyethylene glycol (1-3%) were synthesized and characterized. The physicochemical and biological properties of the polymers and their nanoparticles were evaluated at pH 6.3 and pH 7.4. The degrees of ionization at pH 7.4 were precisely controlled by the composition and increased from 13% for chitosan to 47% for the more substituted derivative. Nanoparticles with very low toxicities and sizes in the range of 100-200 nm, remained stable up to 24 h after their preparation in both the evaluated pHs under plasma osmolality. As probed by scanning electron and confocal microscopies, an efficient cell uptake of spherical nanoparticles mediated a TNF alpha knockdown of almost 60% in RAW 264.7 macrophages, and mRNA silence levels higher than the Lipofectamine (up to 90%) in HeLa cells. Overall, the results showed that these derivatives are promising vectors for in vivo studies under physiological conditions.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionMinistere de l'Economie, de la Science et de l'Innovation du Quebec-
Descrição: dc.descriptionSao Paulo State Univ, Dept Chem & Environm Sci, UNESP, IBILCE, R Cristevao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Phys, UNESP, IBILCE, Sao Jose Do Rio Preto, Brazil-
Descrição: dc.descriptionUniv Montreal, Orthoped Res Lab, Hop Sacre Coeur Montreal, Montreal, PQ, Canada-
Descrição: dc.descriptionSao Paulo State Univ, Dept Chem & Environm Sci, UNESP, IBILCE, R Cristevao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Phys, UNESP, IBILCE, Sao Jose Do Rio Preto, Brazil-
Descrição: dc.descriptionFAPESP: 2017/10331-5-
Descrição: dc.descriptionCAPES: 2017/10331-5-
Descrição: dc.descriptionFAPESP: 2019/27801-0-
Descrição: dc.descriptionFAPESP: 2015/05148-1-
Descrição: dc.descriptionMinistere de l'Economie, de la Science et de l'Innovation du Quebec: PSR-SIIRI-960-
Descrição: dc.descriptionFAPESP: 2009/53989-4-
Formato: dc.format20-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationJournal Of Nanostructure In Chemistry-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectsiRNA-
Palavras-chave: dc.subjectDIPEA-
Palavras-chave: dc.subjectNanoparticles-
Palavras-chave: dc.subjectKnockdown-
Palavras-chave: dc.subjectNon-viral vector-
Palavras-chave: dc.subjectGene therapy-
Palavras-chave: dc.subjectTNF alpha-
Título: dc.titleDouble-grafted chitosans as siRNA nanocarriers: effects of diisopropylethylamine substitution and labile-PEG coating-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.