Bioactivity effects of extracellular matrix proteins on apical papilla cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorLeite, Maria Luisa [UNESP]-
Autor(es): dc.creatorSoares, Diana Gabriela-
Autor(es): dc.creatorAnovazzi, Giovana [UNESP]-
Autor(es): dc.creatorWu Mon, Filipe Koon [UNESP]-
Autor(es): dc.creatorBordini, Ester Alves Ferreira [UNESP]-
Autor(es): dc.creatorHebling, Josimeri [UNESP]-
Autor(es): dc.creatorDe Souza Costa, Carlos Alberto [UNESP]-
Data de aceite: dc.date.accessioned2022-08-04T21:58:50Z-
Data de disponibilização: dc.date.available2022-08-04T21:58:50Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.17632/knzz9hctcd.1-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/218663-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/218663-
Descrição: dc.descriptionPotent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation. Objective: This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs). Methodology: Different concentrations (1, 5, and 10 mu g/mL) of fibronectin (FN), laminin (LM), and type I collagen (COL) were applied to the bottom of non-treated wells of sterilized 96-well plates. Non-treated and pre-treated wells were used as negative (NC) and positive (PC) controls. After seeding the hAPCs (5x10(3) cells/well) on the different substrates, we assessed the following parameters: adhesion, proliferation, spreading, total collagen/type I collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1) (ANOVA/Tukey; alpha=0.05). Results: We observed greater attachment potential for cells on the FN substrate, with the effect depending on concentration. Concentrations of 5 and 10 mu g/mL of FN yielded the highest cell proliferation, spreading and collagen synthesis values with 10 mu g/mL concentration increasing the ITGA5, ITGAV, and COL1A1 expression compared with PC. LM (5 and 10 mu g/mL) showed higher bioactivity values than NC, but those were lower than PC, and COL showed no bioactivity at all. Conclusion: We conclude that FN at 10 mu g/mL concentration exerted the most intense bioactive effects on hAPCs.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Odontol Araraquara, Dept Mat Odontol & Protese, UNESP, Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Odontol Bauru, Dept Dent Endodontia & Mat Odontol, Bauru, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Odontol Araraquara, Dept Morfol & Clin Infantil, UNESP, Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Odontol Araraquara, Dept Fisiol & Patol, UNESP, Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Odontol Araraquara, Dept Mat Odontol & Protese, UNESP, Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Odontol Araraquara, Dept Morfol & Clin Infantil, UNESP, Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Odontol Araraquara, Dept Fisiol & Patol, UNESP, Araraquara, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2017/14210-8-
Descrição: dc.descriptionFAPESP: 2017/22739-9-
Descrição: dc.descriptionCNPq: 302047/2019-0-
Descrição: dc.descriptionCNPq: 408721/2018-9-
Formato: dc.format11-
Idioma: dc.languageen-
Publicador: dc.publisherUniv Sao Paulo Fac Odontologia Bauru-
Relação: dc.relationJournal Of Applied Oral Science-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectGuided tissue regeneration-
Palavras-chave: dc.subjectDental pulp-
Palavras-chave: dc.subjectFibronectin-
Palavras-chave: dc.subjectLaminin-
Palavras-chave: dc.subjectCollagen-
Título: dc.titleBioactivity effects of extracellular matrix proteins on apical papilla cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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