Proteolytic activity of africanized honeybee (Apis mellifera: hymenoptera, apidae) venom

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInstitute of Biosciences of Rio Claro-
Autor(es): dc.creatorLima, P. R. M. De-
Autor(es): dc.creatorBrochetto-braga, M. R. [UNESP]-
Autor(es): dc.creatorChaud-netto, J.-
Data de aceite: dc.date.accessioned2022-02-22T01:13:03Z-
Data de disponibilização: dc.date.available2022-02-22T01:13:03Z-
Data de envio: dc.date.issued2021-07-14-
Data de envio: dc.date.issued2021-07-14-
Data de envio: dc.date.issued2000-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S0104-79302000000100004-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/212339-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/212339-
Descrição: dc.descriptionSome properties of a Africanized honeybee venom proteases were determined by enzymatic assays in solution, electrophoresis in SDS-PAGE, and gel filtration. Bee venom extracts were obtained by reservoir disruption, selective dialysis (cut off 12 kDa) to eliminate small components, such as the protease inhibitor present in the venom, and then fractionation of the dialyzed extract by gel filtration on a Sephadex G-100 column. The optimal conditions for the caseinolytic assays were pH 9.5, 2-hour digestion at 37 °C, and 1% casein concentration. The proteolytic activity was also determined by electrophoresis in SDS-PAGE with co-polymerized gelatin with three major bands of 66.0, 41.6, and 25.1 kDa. A principal serine-protease-like mechanism was revealed in the enriched fraction of proteolytic activity.-
Descrição: dc.descriptionUniversidade Estadual Paulista, Center for the Study of Venoms and Venomous Animals - CEVAP-
Descrição: dc.descriptionInstitute of Biosciences of Rio Claro, Department of Biology-
Descrição: dc.descriptionUniversidade Estadual Paulista, Center of Study of Social Insects-
Descrição: dc.descriptionUniversidade Estadual Paulista, Center for the Study of Venoms and Venomous Animals - CEVAP-
Descrição: dc.descriptionUniversidade Estadual Paulista, Center of Study of Social Insects-
Formato: dc.format64-76-
Idioma: dc.languageen-
Publicador: dc.publisherCentro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP-
Relação: dc.relationJournal of Venomous Animals and Toxins-
Direitos: dc.rightsAcesso aberto-
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Palavras-chave: dc.subjectvenom-
Palavras-chave: dc.subjectafricanized bee-
Palavras-chave: dc.subjectApis mellifera-
Palavras-chave: dc.subjectproteolytic activity-
Palavras-chave: dc.subjectgel filtration chromatography-
Título: dc.titleProteolytic activity of africanized honeybee (Apis mellifera: hymenoptera, apidae) venom-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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