TOXIGENICITY CHARACTERIZATION OF Clostridium perfringens FROM BOVINE ISOLATES

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorInstituto Adolfo Lutz-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorInstituto Biológico de São Paulo-
Autor(es): dc.contributorUNICASTELO-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorBaldassi, L.-
Autor(es): dc.creatorBarbosa, M. L.-
Autor(es): dc.creatorBach, E. E. [UNESP]-
Autor(es): dc.creatorIaria, S. T.-
Data de aceite: dc.date.accessioned2022-02-22T01:10:27Z-
Data de disponibilização: dc.date.available2022-02-22T01:10:27Z-
Data de envio: dc.date.issued2021-07-14-
Data de envio: dc.date.issued2021-07-14-
Data de envio: dc.date.issued2002-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S0104-79302002000100010-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/211532-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/211532-
Descrição: dc.descriptionClinical samples from 71 bovine from different Brazilian states were processed for the analysis of anaerobe organisms with emphasis on the isolation and characterization of Clostridium spp. From these, eighty-nine Clostridium perfringens strains were recovered: 32 from liver, 19 from intestinal contents, 14 from kidney, 6 from rumen, 5 from nervous system, 4 from bone marrow, 2 from udder tract, blood, spleen and lung, and one from muscle. Four reference Clostridium perfringens types A, B, C, and D were used as controls in this study. All isolates were cultivated in appropriate media, and after centrifugation the supernatant and sediment were separated. From pure supernatant post exopolysaccharide (EPS) extraction, mouse toxigenicity tests were performed, determining protein and protein plus carbohydrate, respectively. ELISA was performed from sediments. The results showed that 51 (57.3%) of the isolates were toxigenic to mice when inoculated by intraperitoneal route; bacteria from different organs had variable patterns of toxigenicity. Toxigenicity of EPS extracts was only expressed when protein concentration was 0.04 mg/mL and between 0.31 and 0.5 mg/mL for carbohydrate. Isolates were characterized as toxigenic when showing optimum protein and carbohydrate concentrations.-
Descrição: dc.descriptionInstituto Adolfo Lutz-
Descrição: dc.descriptionUniversidade de São Paulo, Instituto de Ciências Biomedicina-
Descrição: dc.descriptionInstituto Biológico de São Paulo, Laboratório de Bacteriologia Geral e Micobacterioses-
Descrição: dc.descriptionUNICASTELO, Departamento de Química-
Descrição: dc.descriptionUniversidade Estadual Paulista-
Descrição: dc.descriptionUniversidade Estadual Paulista-
Formato: dc.format112-126-
Idioma: dc.languageen-
Publicador: dc.publisherCentro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP-
Relação: dc.relationJournal of Venomous Animals and Toxins-
Direitos: dc.rightsAcesso aberto-
???dc.source???: dc.sourceSciELO-
Palavras-chave: dc.subjectClostridium perfringens-
Palavras-chave: dc.subjecttoxigenicity-
Palavras-chave: dc.subjectbovine isolates-
Palavras-chave: dc.subjectELISA-
Título: dc.titleTOXIGENICITY CHARACTERIZATION OF Clostridium perfringens FROM BOVINE ISOLATES-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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