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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Federal de São Carlos (UFSCar) | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Zanesco-Fontes, Ideli | - |
Autor(es): dc.creator | Lopes Silva, Ana Carolina [UNESP] | - |
Autor(es): dc.creator | Silva, Patricia Bento da [UNESP] | - |
Autor(es): dc.creator | Duarte, Jonatas Lobato [UNESP] | - |
Autor(es): dc.creator | Di Filippo, Leonardo Delello [UNESP] | - |
Autor(es): dc.creator | Chorilli, Marlus [UNESP] | - |
Autor(es): dc.creator | Cominetti, Marcia Regina | - |
Autor(es): dc.creator | Baptista Moreno Martin, Ana Carolina | - |
Data de aceite: dc.date.accessioned | 2022-02-22T01:08:15Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T01:08:15Z | - |
Data de envio: dc.date.issued | 2021-06-26 | - |
Data de envio: dc.date.issued | 2021-06-26 | - |
Data de envio: dc.date.issued | 2021-05-18 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1208/s12249-021-02006-w | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/210813 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/210813 | - |
Descrição: dc.description | The apoptotic, cytotoxic, and cytostatic activities for [10]-gingerol in triple-negative breast cancer cells (TNBCs) were already reported. However, despite these important antitumor activities, the compound has the disadvantage to have a hydrophobic characteristic, hindering in vivo administration. To surpass this issue, in this study we have created a [10]-gingerol-loaded nanoemulsion (10GNE) in order to increase the stability and solubility of the compound. The nanoemulsion was characterized and tested for its cytotoxic, cytostatic, and apoptotic effects on a panel of murine and human TNBC cell lines, as well as non-tumor cells, and compared with a [10]-gingerol-free nanoemulsion (NE) and with [10]-gingerol itself. Except for the murine 4T1.13 cell line, the IC50 of the free 10G molecule, after 72 h of incubation, was higher in all cell lines tested, both murine and human, demonstrating therefore the efficacy of the 10GNE regarding cytotoxicity. In murine tumor cells, 60 mu M 10GNE was able to arrest cell cycle at sub-G0 phase and induce apoptosis, leading to 48% and 78% of total cell death in 4T1.13 and 4T1Br4 murine tumor cells, respectively. This represents an improvement compared to 10G-free molecule that only induced 74% of total apoptosis at 100 mu M in 4T1Br4 cells. Taken together, our results show that nanoformulation preserved the [10]-gingerol cytotoxic and cytostatic properties and improved its apoptotic function on murine TNBC cell lines. These data open new perspectives to a more suitable drug-delivery approach for [10]-gingerol for TNBC treatment that should be further demonstrated using in vivo assays. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Univ Fed Sao Carlos, Dept Gerontol, Rodovia Washington Luis,Km 235, BR-13565905 Sao Carlos, SP, Brazil | - |
Descrição: dc.description | Sao Paulo State Univ, Sch Pharmaceut Sci, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP, Brazil | - |
Descrição: dc.description | Sao Paulo State Univ, Sch Pharmaceut Sci, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP, Brazil | - |
Descrição: dc.description | FAPESP: 2013/07600-3 | - |
Descrição: dc.description | FAPESP: 2016/23202-6 | - |
Descrição: dc.description | FAPESP: 2018/17756-4 | - |
Formato: dc.format | 8 | - |
Idioma: dc.language | en | - |
Publicador: dc.publisher | Springer | - |
Relação: dc.relation | Aaps Pharmscitech | - |
???dc.source???: dc.source | Web of Science | - |
Palavras-chave: dc.subject | [10]-gingerol | - |
Palavras-chave: dc.subject | formulation | - |
Palavras-chave: dc.subject | nanoemulsion | - |
Palavras-chave: dc.subject | triple-negative breast cancer | - |
Título: dc.title | [10]-Gingerol-Loaded Nanoemulsion and its Biological Effects on Triple-Negative Breast Cancer Cells | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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