[10]-Gingerol-Loaded Nanoemulsion and its Biological Effects on Triple-Negative Breast Cancer Cells

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Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorZanesco-Fontes, Ideli-
Autor(es): dc.creatorLopes Silva, Ana Carolina [UNESP]-
Autor(es): dc.creatorSilva, Patricia Bento da [UNESP]-
Autor(es): dc.creatorDuarte, Jonatas Lobato [UNESP]-
Autor(es): dc.creatorDi Filippo, Leonardo Delello [UNESP]-
Autor(es): dc.creatorChorilli, Marlus [UNESP]-
Autor(es): dc.creatorCominetti, Marcia Regina-
Autor(es): dc.creatorBaptista Moreno Martin, Ana Carolina-
Data de aceite: dc.date.accessioned2022-02-22T01:08:15Z-
Data de disponibilização: dc.date.available2022-02-22T01:08:15Z-
Data de envio: dc.date.issued2021-06-26-
Data de envio: dc.date.issued2021-06-26-
Data de envio: dc.date.issued2021-05-18-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1208/s12249-021-02006-w-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/210813-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/210813-
Descrição: dc.descriptionThe apoptotic, cytotoxic, and cytostatic activities for [10]-gingerol in triple-negative breast cancer cells (TNBCs) were already reported. However, despite these important antitumor activities, the compound has the disadvantage to have a hydrophobic characteristic, hindering in vivo administration. To surpass this issue, in this study we have created a [10]-gingerol-loaded nanoemulsion (10GNE) in order to increase the stability and solubility of the compound. The nanoemulsion was characterized and tested for its cytotoxic, cytostatic, and apoptotic effects on a panel of murine and human TNBC cell lines, as well as non-tumor cells, and compared with a [10]-gingerol-free nanoemulsion (NE) and with [10]-gingerol itself. Except for the murine 4T1.13 cell line, the IC50 of the free 10G molecule, after 72 h of incubation, was higher in all cell lines tested, both murine and human, demonstrating therefore the efficacy of the 10GNE regarding cytotoxicity. In murine tumor cells, 60 mu M 10GNE was able to arrest cell cycle at sub-G0 phase and induce apoptosis, leading to 48% and 78% of total cell death in 4T1.13 and 4T1Br4 murine tumor cells, respectively. This represents an improvement compared to 10G-free molecule that only induced 74% of total apoptosis at 100 mu M in 4T1Br4 cells. Taken together, our results show that nanoformulation preserved the [10]-gingerol cytotoxic and cytostatic properties and improved its apoptotic function on murine TNBC cell lines. These data open new perspectives to a more suitable drug-delivery approach for [10]-gingerol for TNBC treatment that should be further demonstrated using in vivo assays.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniv Fed Sao Carlos, Dept Gerontol, Rodovia Washington Luis,Km 235, BR-13565905 Sao Carlos, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Sch Pharmaceut Sci, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Sch Pharmaceut Sci, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2013/07600-3-
Descrição: dc.descriptionFAPESP: 2016/23202-6-
Descrição: dc.descriptionFAPESP: 2018/17756-4-
Formato: dc.format8-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationAaps Pharmscitech-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subject[10]-gingerol-
Palavras-chave: dc.subjectformulation-
Palavras-chave: dc.subjectnanoemulsion-
Palavras-chave: dc.subjecttriple-negative breast cancer-
Título: dc.title[10]-Gingerol-Loaded Nanoemulsion and its Biological Effects on Triple-Negative Breast Cancer Cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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