Statherin-derived peptide protects against intrinsic erosion

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorTaira, Even Akemi-
Autor(es): dc.creatorCarvalho, Gabriel-
Autor(es): dc.creatorFerrari, Carolina Ruis-
Autor(es): dc.creatorMartini, Tatiana-
Autor(es): dc.creatorPela, Vinicius Taioqui-
Autor(es): dc.creatorOliveira Ventura, Talita Mendes-
Autor(es): dc.creatorDionizio, Aline Salgado-
Autor(es): dc.creatorCrusca, Edson [UNESP]-
Autor(es): dc.creatorMarchetto, Reinaldo [UNESP]-
Autor(es): dc.creatorRabelo Buzalaf, Marilia Afonso-
Data de aceite: dc.date.accessioned2022-02-22T01:01:31Z-
Data de disponibilização: dc.date.available2022-02-22T01:01:31Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2020.104890-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/210511-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/210511-
Descrição: dc.descriptionObjectives: In the present study, we used an in vitro initial intrinsic erosion model to evaluate: (experiment 1) the influence of the degree of serine (Ser) phosphorylation of peptides containing the 15 N-terminal residues of statherin and (experiment 2) the effect of different concentrations of the peptide with the best performance in experiment 1 on initial enamel erosion. Design: Bovine enamel specimens were divided into 6 groups (n = 15/group) for each experiment. In experiment 1, the peptides evaluated (at 1.88 x 10(-5) M) were: not phosphorylated (StatSS), phosphorylated in Ser2 (StatpSS), phosphorylated in Ser3 (StatSpS) phosphorylated in Ser2 and Ser3 (StatpSpS). Phosphate buffer and human recombinant statherin were used as negative and positive controls, respectively. In experiment 2, StatpSpS was evaluated at different concentrations: 0.94, 1.88, 3.76 and 7.52 x 10(-5) M. Phosphate buffer and 0.1 mg/mL CaneCPI-5 were employed as negative and positive controls, respectively. In each experiment, the specimens were incubated with the solutions for 2 h, then the AEP was allowed to form (under human pooled saliva) for 2 h. The specimens were then challenged with 0.01 M HCl for 10 s. Demineralization was evaluated by percentage of surface hardness change (%SHC). Data were analyzed by ANOVA and Tukey's test (p < 0.05). Results: In experiment 1, only StatpSpS significantly reduced the % SHC in comparison with control. In experiment 2, 1.88 x 10(-5) M StatpSpS significantly reduced the %SHC in comparison with control. Conclusions: This is the first study showing that statherin-derived peptide might protect against intrinsic erosion.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniv Sao Paulo, Bauru Sch Dent, Bauru, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Chem Inst, Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Chem Inst, Araraquara, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2018/18749-1-
Formato: dc.format5-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationArchives Of Oral Biology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectAcquired pellicle-
Palavras-chave: dc.subjectStatherin-
Palavras-chave: dc.subjectEnamel-
Palavras-chave: dc.subjectDental erosion-
Palavras-chave: dc.subjectProteomics-
Título: dc.titleStatherin-derived peptide protects against intrinsic erosion-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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