Antinociception induced by artemisinin nanocapsule in a model of postoperative pain via spinal TLR4 inhibition

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniv Fed Alfenas-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorElisei, L. M. S.-
Autor(es): dc.creatorMoraes, T. R.-
Autor(es): dc.creatorMalta, I. H.-
Autor(es): dc.creatorCharlie-Silva, I.-
Autor(es): dc.creatorSousa, I. M. O.-
Autor(es): dc.creatorVeras, F. P.-
Autor(es): dc.creatorFoglio, M. A.-
Autor(es): dc.creatorFraceto, L. F. [UNESP]-
Autor(es): dc.creatorGaldino, G.-
Data de aceite: dc.date.accessioned2022-02-22T01:00:04Z-
Data de disponibilização: dc.date.available2022-02-22T01:00:04Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-09-16-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10787-020-00756-w-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/210454-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/210454-
Descrição: dc.descriptionArtemisinin (ART) was initially described for the control of inflammation and pain. However, the mechanisms involved with its antinociceptive effect are still poorly understood. Thus, this present study aimed to investigate the effect of ART in both free and nanocapsulated form on postoperative pain, as well as the participation of the spinal Toll-like receptor 4 (TLR4) in this process. Postoperative pain was induced using the skin/muscle incision retraction (SMIR) model in male Swiss mice. After 3 and 28 days of SMIR, the animals received an intrathecal injection of free or nanocapsulated ART, and the nociceptive threshold was evaluated by von Frey filament test. To evaluate the involvement of the microglia, astrocytes, and TLR4, minocycline (a microglia inhibitor), fluorocitrate (an astrocyte inhibitor), andLipopolysaccharide Rhodobacter sphaeroides(LPS-RS), a TLR4 antagonist, were intrathecally injected on the third day of SMIR. The levels of spinal TLR4 protein and proinflammatory cytokines tumor necrosis factor-alpha (TNF-alpha), and interleukin-1-beta (IL-1 beta) were quantified by western blot and enzyme-linked immunosorbent assay, respectively. The results showed that free ART reduced postoperative pain (P < 0.001,F-5,F-30 = 7.49, 16.66% for 1000 ng dose; andP < 0.01,F-5,F-30 = 7.49, 14.58% for 500 ng dose) on the 3rd day of SMIR; while the ART nanocapsule had this effect on both the third (P < 0.001;F-5,F-30 = 4.94; 43.75, 39.58 and 72.91% for the 250, 500 and 1000 ng doses, respectively) and 28th (P < 0.05;F-5,F-30 = 7.71; 29.16 and 33.33% for the 500 and 1000 ng doses, respectively) day. The ART nanocapsule had a more potent and longer antinociceptive effect than free ART or morphine. Postoperative pain was also reduced by minocycline and LPS-RS. The ART nanocapsule also reduced the increased levels of TLR4, TNF-alpha, and IL-1 beta induced by SMIR. These data suggest that the ART nanocapsule has a potent analgesic effect on postoperative pain at the spinal level, and this response involves the inhibition of TLR4 and the proinflammatory cytokines TNF-alpha and IL-1 beta.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniv Fed Alfenas, Fac Motr Sci, Sci Motr Inst, Jovino Fernandes Sales Ave 2600, BR-37133840 Alfenas, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Dept Pharmacol, Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Estadual Campinas, Fac Pharmaceut Sci, Campinas, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Sci & Technol, Sorocaba, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Sci & Technol, Sorocaba, Brazil-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2014/16008-3-
Formato: dc.format1537-1551-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationInflammopharmacology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectArtemisinin-
Palavras-chave: dc.subjectPostoperative pain-
Palavras-chave: dc.subjectSpinal cord-
Palavras-chave: dc.subjectToll-like receptor 4-
Palavras-chave: dc.subjectProinflammatory cytokines-
Título: dc.titleAntinociception induced by artemisinin nanocapsule in a model of postoperative pain via spinal TLR4 inhibition-
Tipo de arquivo: dc.typelivro digital-
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