Acquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniv Bern-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorPela, Vinicius Taioqui-
Autor(es): dc.creatorRabelo Buzalaf, Marilia Afonso-
Autor(es): dc.creatorNiemeyer, Samira Helena-
Autor(es): dc.creatorBaumann, Tommy-
Autor(es): dc.creatorHenrique-Silva, Flavio-
Autor(es): dc.creatorToyama, Danyelle-
Autor(es): dc.creatorCrusca, Edson [UNESP]-
Autor(es): dc.creatorMarchetto, Reinaldo [UNESP]-
Autor(es): dc.creatorLussi, Adrian-
Autor(es): dc.creatorCarvalho, Thiago Saads-
Data de aceite: dc.date.accessioned2022-02-22T00:59:53Z-
Data de disponibilização: dc.date.available2022-02-22T00:59:53Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2021.103612-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/210426-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/210426-
Descrição: dc.descriptionObjective: This study investigated the mechanism of action of different proteins/peptides (separately or in combination), focusing on how they act directly on the native enamel surface and on modifying the salivary pellicle. Methods: A total of 170 native human enamel specimens were prepared and submitted to different treatments (2 h; 37 degrees C): with deionized water, CaneCPI-5, Hemoglobin, Statherin, or a combination of all three proteins/peptides. The groups were subdivided into treatment acting on the enamel surface (NoP - absence of salivary pellicle), and treatment modifying the salivary pellicle (P). Treatment was made (2 h; 37 degrees C) in all specimens, and later, for P, the specimens were incubated in human saliva (2 h; 37 degrees C). In both cases, the specimens were immersed in 1% citric acid (pH 3.6; 2 min; 25 degrees C). Calcium released from enamel (CaR) and its relative surface reflection intensity (%SRI) was measured after 5 cycles. Between-group differences were verified with two-way ANOVA, with presence of pellicle and treatment as factors (alpha = 0.05). Results: The presence of pellicle provided better protection regarding %SRI (p < 0.01), but not regarding CaR (p = 0.201). In relation to treatment, when compared to the control group, all proteins/peptides provided significantly better protection (p < 0.01 for %SRI and Car). The combination of all three proteins/peptides demonstrated the best protective effect (p < 0.01 for %SRI). Conclusion: Depending on the protein or peptide, its erosion-inhibiting effect derives from their interaction with the enamel surface or from modifying the pellicle, so a combination of proteins and peptides provides the best protection. Clinical significance: The present study opens a new direction for a possible treatment with a combination of proteins for native human enamel, which can act directly on the enamel surface as well on the modification of the salivary pellicle, for the prevention of dental erosion.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniv Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Bauru Sch Dent, Dept Biol Sci, SP Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP, Brazil-
Descrição: dc.descriptionUniv Bern, Sch Dent Med, Dept Restorat Prevent & Pediat Dent, Bern, Switzerland-
Descrição: dc.descriptionUniv Estadual Paulista, Chem Inst, Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Chem Inst, Araraquara, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2019/08437-5-
Formato: dc.format7-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationJournal Of Dentistry-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectAcquired pellicle-
Palavras-chave: dc.subjectDental erosion-
Palavras-chave: dc.subjectEnamel-
Palavras-chave: dc.subjectProtein-
Palavras-chave: dc.subjectPellicle modification-
Palavras-chave: dc.subjectsaliva-
Título: dc.titleAcquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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