EVALUATION OF THE FREEZABILITY OF THE BOVINE EPIDIDYMIS TAIL SPERM WITH THE ADDITION OF ANTIOXIDANTS

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Autor(es): dc.contributorUniv Paranaense-
Autor(es): dc.contributorUniv Santo Amaro-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.creatorCruz, Gabriela Passamani da-
Autor(es): dc.creatorZanfrilli dos Santos, Ana Paula-
Autor(es): dc.creatorFreitas Guaitolini, Carlos Renato de-
Autor(es): dc.creatorDenck Tramontin, Marcio Luiz-
Autor(es): dc.creatorRigoto, Renata Patricia-
Autor(es): dc.creatorCrespilho, Andre Maciel-
Autor(es): dc.creatorFreitas Dell'Aqua, Camila de Paula [UNESP]-
Autor(es): dc.creatorMello Martins, Maria Isabel-
Autor(es): dc.creatorMarques, Ana Beatriz-
Autor(es): dc.creatorTsunokawa Hidalgo, Myrian Megumy-
Autor(es): dc.creatorOliveira Sestari, Danielle Andressa-
Autor(es): dc.creatorMagalhales, Ricardo-
Autor(es): dc.creatorDias Maziero, Rosiara Rosaria-
Data de aceite: dc.date.accessioned2022-02-22T00:59:08Z-
Data de disponibilização: dc.date.available2022-02-22T00:59:08Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-03-01-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/210193-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/210193-
Descrição: dc.descriptionBACKGROUND: The cryopreservation and recovery of epididymis tail sperm is an important biotechnology dependent on the composition of the freezing medium. OBJETIVE: To evaluate the effect of melatonin, added to commercial freezing medium extender, on the kinetics and viability of bovine epididymis tail sperm. MATERIAL AND METHODS: Five routines were performed, each consisting of eight epididymis and the structures were sliced onto a glass plate containing a commercial diluting medium for Botubov (R). The samples were divided into four groups, with 80 x 10(6) spermatozoa per mL. Group 1: samples diluted in Botubov (R). Group 2: samples centrifuged (600 g, 10 min), and the pellet re-suspended in Botubov (R). Group 3, samples diluted in Botubov (R) containing 100 pM melatonin. Group 4: samples centrifuged (600 g, 10 min) and the pellet resuspended in Botubov (R) with 100 pM melatonin. The samples were transferred to 0.5 mL straws at 40 x 10(6) viable spermatozoa, stabilized at 5 degrees C for 4 h, transferred to liquid nitrogen vapour for 20 min, dipped in liquid nitrogen and stored in a cryogenic cylinder. After thawing (46 degrees C, 15 s), sperm kinetics and viability parameters were evaluated. RESULTS: There was no difference in the parameters of total motility (MT, %), progressive motility (MP, %), progressive linear velocity (VSL, mu m/s), curvilinear velocity (VCL, mu m/s), linearity (LIN, %), spermatozoa with rapid movement (RAP, %) and level of intact plasma membranes and acrosome (IPMA, %) among the groups studied. However, a difference was observed between the routines performed. CONCLUSION: The protocol for freezing bovine epididymis tail sperm is applicable; however, there is an influence of the epididymis used, for the best efficacy of this biotechnology.-
Descrição: dc.descriptionUniv Paranaense, Umuarama, PR, Brazil-
Descrição: dc.descriptionUniv Santo Amaro, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionFMVZ UNESP, Dept Radiol & Anim Reprod, Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Londrina, UEL, Londrina, Parana, Brazil-
Descrição: dc.descriptionFMVZ UNESP, Dept Radiol & Anim Reprod, Botucatu, SP, Brazil-
Formato: dc.format81-86-
Idioma: dc.languageen-
Publicador: dc.publisherCryo Letters-
Relação: dc.relationCryoletters-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectbovine epididymis-
Palavras-chave: dc.subjectfreezing-
Palavras-chave: dc.subjectmelatonin-
Palavras-chave: dc.subjectspermatic parameters-
Título: dc.titleEVALUATION OF THE FREEZABILITY OF THE BOVINE EPIDIDYMIS TAIL SPERM WITH THE ADDITION OF ANTIOXIDANTS-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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