A peptide-based coating toolbox to enable click chemistry on polymers, metals, and silicon through sortagging

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorRhein Westfal TH Aachen-
Autor(es): dc.contributorDWI Leibniz Inst Interact Mat-
Autor(es): dc.contributorAssiut Univ-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorNoeth, Maximilian-
Autor(es): dc.creatorZou, Zhi-
Autor(es): dc.creatorEl-Awaad, Islam-
Autor(es): dc.creatorLencastre Novaes, Leticia C. de-
Autor(es): dc.creatorDilarri, Guilherme [UNESP]-
Autor(es): dc.creatorDavari, Mehdi D.-
Autor(es): dc.creatorFerreira, Henrique [UNESP]-
Autor(es): dc.creatorJakob, Felix-
Autor(es): dc.creatorSchwaneberg, Ulrich-
Data de aceite: dc.date.accessioned2022-02-22T00:58:14Z-
Data de disponibilização: dc.date.available2022-02-22T00:58:14Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-02-03-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/bit.27666-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/210003-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/210003-
Descrição: dc.descriptionA versatile peptide-based toolbox for surface functionalization was established by a combination of a universal material binding peptide (LCI-anchor peptide) and sortase-mediated bioconjugation (sortagging). This toolbox facilitates surface functionalization either as a one- or a two-step strategy. In the case of the one-step strategy, the desired functionality was directly introduced to LCI. For the two-step strategy, LCI was modified with a reactive group, which can be further functionalized (e.g., employing click chemistry). Sortagging of LCI, employing sortase A from Staphylococcus aureus, was achieved with six different amine compounds: dibenzocyclooctyne amine, biotin-polyethylene glycol amine, Cyanine-3 amine, methoxypolyethylene glycol amine (Mn = 5000 Da), and 2,2,3,3,4,4,4-Heptafluorobutylamine. The purification of LCI-amine sortagging products was performed by a negative purificationusing Strep-tag II affinity chromatography, resulting in LCI-amine conjugates with purities >90%. For the two-step strategy, the LCI-dibenzocyclooctyne sortagging product was purified and enabled, through copper-free azide-alkyne click chemistry, universal surface functionalization of material surfaces such as polypropylene, polyethylene terephthalate, stainless steel, gold, and silicon. The click reaction was performed before or after surface binding of LCI-dibenzocyclooctyne. Finally, in the case of the one-step strategy, polypropylene was directly functionalized with Cyanine-3 and biotin-polyethylene glycol amine.-
Descrição: dc.descriptionChinese Scholarship Council-
Descrição: dc.descriptionBundesministerium fur Bildung und Forschung-
Descrição: dc.descriptionCenter for Chemical Polymer Technology CPT-
Descrição: dc.descriptionRhein Westfal TH Aachen, Lehrstuhl Biotechnol, Worringerweg 3, D-52074 Aachen, Germany-
Descrição: dc.descriptionDWI Leibniz Inst Interact Mat, Forckenbeckstr 50, D-52074 Aachen, Germany-
Descrição: dc.descriptionAssiut Univ, Dept Pharmacognosy, Fac Pharm, Assiut, Egypt-
Descrição: dc.descriptionSao Paulo State Univ, Biosci Inst, Dept Appl & Gen Biol, Rio Claro, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Biosci Inst, Dept Appl & Gen Biol, Rio Claro, SP, Brazil-
Descrição: dc.descriptionChinese Scholarship Council: 201406740053-
Descrição: dc.descriptionBundesministerium fur Bildung und Forschung: FKZ 031B0384-
Descrição: dc.descriptionCenter for Chemical Polymer Technology CPT: EFRE 300088302-
Formato: dc.format1520-1530-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-Blackwell-
Relação: dc.relationBiotechnology And Bioengineering-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectadhesion promoter-
Palavras-chave: dc.subjectanchor peptide-
Palavras-chave: dc.subjectclick chemistry-
Palavras-chave: dc.subjectsortase&#8208-
Palavras-chave: dc.subjectmediated ligation-
Palavras-chave: dc.subjectsurface functionalization-
Título: dc.titleA peptide-based coating toolbox to enable click chemistry on polymers, metals, and silicon through sortagging-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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