Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorTexas A&M Univ-
Autor(es): dc.contributorUniv Western Sao Paulo-
Autor(es): dc.creatorDias, H. P. [UNESP]-
Autor(es): dc.creatorPoole, R. K.-
Autor(es): dc.creatorAlbuquerque, J. P. [UNESP]-
Autor(es): dc.creatorSantos, P. H. dos [UNESP]-
Autor(es): dc.creatorCastilho, A. C. S.-
Autor(es): dc.creatorPohler, K. G.-
Autor(es): dc.creatorVasconcelos, J. L. M. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:58:04Z-
Data de disponibilização: dc.date.available2022-02-22T00:58:04Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2020.106681-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209952-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209952-
Descrição: dc.descriptionThe objective was to investigate effects of progesterone (P-4) dose on abundance of luteinizing hormone receptor (LHCGR), aromatase (CYP19A1), 3 beta-hydroxysteroid dehydrogenase (HSD3B1), and other steroidogenic mRNA transcripts in granulosa cells from dominant follicles. Nellore heifers were assigned to one of six groups: new, first-use controlled internal drug release device (CIDR1) inserted for 5 days (Large-P-4-dose-D5; n = 7) or 6 days (Large-P-4-dose-D6; n = 8), prostaglandin (PG)F-2 alpha administered on DO and 1 previously-used CIDR (CIDR3) inserted for 5 days (Small-P-4-dose-D5; n = 8) or 6 days (Small-P-4-dose-D6; n = 8), CIDR1 inserted on DO and removed plus PGF(2 alpha) on D5 (Large-P-4-dose-pmestrus (PE); n = 7), and CIDR3 and PGF(2 alpha) on DO and 1, CIDR3 removed plus PGF(2 alpha) on D5 (Small-P-4-dose-PE; n = 7). Duration of P-4 treatment (D5 compared to D6) affected abundances of CYP19A1 mRNA transcripts, with there being greater abundances on D6 than D5 (P <= 0.05). Heifers treated with the large dose of P-4 had a smaller dominant follicle, less serum and intra-follicular estradiol (E-2) concentrations (P <= 0.05) and lesser LHCGR, CYP19A1, and HSD3B1 transcript abundances (P <= 0.05). Heifers treated to induce PE had a larger follicle diameter (P = 0.09), greater intra-follicular E-2 concentrations and larger abundances of CYP19A1 mRNA transcript (P <= 0.05) than heifers of the D6 group. Overall, treatment with larger doses of P-4 resulted in lesser abundances of LHCGR, HSD3B1, and CYP19A1 mRNA transcripts; thus, potentially leading to development of smaller dominant follicles and lesser E-2 concentrations.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUSDA-NIFAHatch/Multistate Project-
Descrição: dc.descriptionSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, Brazil-
Descrição: dc.descriptionTexas A&M Univ, Dept Anim Sci, Pregnancy & Dev Programming Area Excellence, College Stn, TX 77843 USA-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci, BR-18168000 Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Western Sao Paulo, Presidente Prudente, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci, BR-18168000 Botucatu, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2013/17181-8-
Descrição: dc.descriptionUSDA-NIFAHatch/Multistate Project: W3112-TEX07702-
Formato: dc.format10-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationAnimal Reproduction Science-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectLH receptor-
Palavras-chave: dc.subjectProgesterone-
Palavras-chave: dc.subjectGranulosa cell-
Palavras-chave: dc.subjectNellore heifers-
Título: dc.titleProgesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.