GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniv Paranaense-
Autor(es): dc.contributorOeste Paulista Univ-
Autor(es): dc.contributorJose do Rosario Vellano Univ-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorFreitas Guaitolini, Carlos Renato de-
Autor(es): dc.creatorDias Maziero, Rosiara Rosaria-
Autor(es): dc.creatorSouza Castilho, Anthony Cesar de-
Autor(es): dc.creatorPagnano Derussi, Ana Augusta-
Autor(es): dc.creatorVolpato, Rodrigo [UNESP]-
Autor(es): dc.creatorAckermann, Camila Louise [UNESP]-
Autor(es): dc.creatorMatsubara, Lidia Mitsuko [UNESP]-
Autor(es): dc.creatorLopes, Maria Denise [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:58:04Z-
Data de disponibilização: dc.date.available2022-02-22T00:58:04Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-10-31-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209949-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209949-
Descrição: dc.descriptionBACKGROUND: Canine embryo cryopreservation and subsequent transfer are relevant in the use of reproductive technologies. OBJECTIVE: The purpose of this study is the identification and quantification of the gene expression BAX and Bcl2, AQP3, Na+/K+ ATPase alpha 1 and beta 1 and LIFr in canine embryos obtained in vivo and after freezing. MATERIALS AND METHODS: For the collection of embryos, the bitches were identified at pro-estrous until the detection of 80-90% superficial cells. After that, they were artificially inseminated with fresh semen. The embryos were collected after ovariohysterectomy. RNA was extracted and amplified, and embryos were randomly distributed into fresh (Fr) and frozen/thawed (Ft) groups. RESULTS: Eighteen blastocysts were collected from three bitches. Genes BAX, AQP3 and LIFr did not differ among the studied groups. CONCLUSION: We suggest, through these results, that the genes BAX, Bcl2, AQP3, Na + / K + ATPase alpha 1 and beta 1 and LIFr were expressed in canine blastocysts collected in vivo and after slow freezing cryopreservation.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniv Paranaense, Umuarama, PR, Brazil-
Descrição: dc.descriptionOeste Paulista Univ, Presidente Prudente, SP, Brazil-
Descrição: dc.descriptionJose do Rosario Vellano Univ, Alfenas, MG, Brazil-
Descrição: dc.descriptionFMVZ UNESP, Dept Radiol & Anim Reprod, Botucatu, SP, Brazil-
Descrição: dc.descriptionFMVZ UNESP, Dept Radiol & Anim Reprod, Botucatu, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2011/08798-6-
Descrição: dc.descriptionFAPESP: 2012/03912-8-
Formato: dc.format330-336-
Idioma: dc.languageen-
Publicador: dc.publisherCryo Letters-
Relação: dc.relationCryoletters-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectgenes-
Palavras-chave: dc.subjectembryos-
Palavras-chave: dc.subjectslow freezing-
Título: dc.titleGENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.