In Vitro Induction of Pluripotency from Equine Fibroblasts in 20% or 5% Oxygen

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniv Montreal-
Autor(es): dc.creatorCastro, Raquel V. G. de [UNESP]-
Autor(es): dc.creatorPieri, Naira C. G.-
Autor(es): dc.creatorFantinato Neto, Paulo-
Autor(es): dc.creatorGrizendi, Bianca M.-
Autor(es): dc.creatorDoria, Renata G. S.-
Autor(es): dc.creatorMeirelles, Flavio V.-
Autor(es): dc.creatorSmith, Lawrence C.-
Autor(es): dc.creatorGarcia, Joaquim M. [UNESP]-
Autor(es): dc.creatorBressan, Fabiana F.-
Data de aceite: dc.date.accessioned2022-02-22T00:58:03Z-
Data de disponibilização: dc.date.available2022-02-22T00:58:03Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-25-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1155/2020/8814989-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209944-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209944-
Descrição: dc.descriptionThe cellular reprogramming into pluripotency is influenced by external and internal cellular factors, such as in vitro culture conditions (e.g., environmental oxygen concentration), and the aging process. Herein, we aimed to generate and maintain equine iPSCs (eiPSCs) derived from fibroblasts of a horse older than 20 years and to evaluate the effect of different levels of oxygen tension (atmospheric 20% O-2, 5% O-2, or 20% to 5% O-2) on these cells. Fibroblasts were reprogrammed, and putative eiPSCs were positive for positive alkaline phosphatase detection; they were positive for pluripotency-related genes OCT4, REX1, and NANOG; immunofluorescence-positive staining was presented for OCT4 and NANOG (all groups), SOX2 (groups 5% O-2 and 20% to 5% O-2), and TRA-1-60, TRA-1-81, and SSEA-1 (only in 20% O-2); they formed embryoid bodies; and there is spontaneous differentiation in mesoderm, endoderm, and ectoderm embryonic germ layers. In addition to the differences in immunofluorescence analysis results, the eiPSC colonies generated at 20% O-2 presented a more compact morphology with a well-defined border than cells cultured in 5% O-2 and 20% to 5% O-2. Significant differences were also observed in the expression of genes related to glucose metabolism, mitochondrial fission, and hypoxia (GAPDH, GLUT3, MFN1, HIF1 alpha, and HIF2 alpha), after reprogramming. Our results show that the derivation of eiPSCs was not impaired by aging. Additionally, this study is the first to compare high and low oxygen cultures of eiPSCs, showing the generation of pluripotent cells with different profiles. Under the tested conditions, the lower oxygen tension did not favor the pluripotency of eiPSCs. This study shows that the impact of oxygen atmosphere has to be considered when culturing eiPSCs, as this condition influences the pluripotency characteristics.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSao Paulo State Univ, Fac Agr & Vet Sci, Dept Pathol Reprod & One Hlth, BR-14884900 Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Sao Paulo FZEA USP, Fac Anim Sci & Food Engn, Dept Vet Med, BR-13635900 Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Sao Paulo FMVZ USP, Sch Vet Med & Anim Sci, Dept Anim Reprod, BR-13635900 Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Montreal, Fac Vet Med, Ctr Rech Reprod & Fertilite, Montreal, PQ J2S 2M2, Canada-
Descrição: dc.descriptionSao Paulo State Univ, Fac Agr & Vet Sci, Dept Pathol Reprod & One Hlth, BR-14884900 Sao Paulo, Brazil-
Descrição: dc.descriptionFAPESP: 2015/26818-5-
Descrição: dc.descriptionFAPESP: 2018/04009-6-
Descrição: dc.descriptionFAPESP: 2013/08135-2-CEPID-CTC-
Formato: dc.format16-
Idioma: dc.languageen-
Publicador: dc.publisherHindawi Ltd-
Relação: dc.relationStem Cells International-
???dc.source???: dc.sourceWeb of Science-
Título: dc.titleIn Vitro Induction of Pluripotency from Equine Fibroblasts in 20% or 5% Oxygen-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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