Sugarcane mosaic virus mediated changes in cytosine methylation pattern and differentially transcribed fragments in resistance-contrasting sugarcane genotypes

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorInst Agron-
Autor(es): dc.creatorSilva, Marcel Fernando da [UNESP]-
Autor(es): dc.creatorGoncalves, Marcos Cesar-
Autor(es): dc.creatorBrito, Michael dos Santos-
Autor(es): dc.creatorMedeiros, Cibele Nataliane-
Autor(es): dc.creatorHarakava, Ricardo-
Autor(es): dc.creatorAndrade Landell, Marcos Guimaraes de-
Autor(es): dc.creatorPinto, Luciana Rossini-
Data de aceite: dc.date.accessioned2022-02-22T00:57:21Z-
Data de disponibilização: dc.date.available2022-02-22T00:57:21Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-08-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0241493-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209687-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209687-
Descrição: dc.descriptionSugarcane mosaic virus (SCMV) is the causal agent of sugarcane mosaic disease (SMD) in Brazil; it is mainly controlled by using resistant cultivars. Studies on the changes in sugarcane transcriptome provided the first insights about the molecular basis underlying the genetic resistance to SMD; nonetheless, epigenetic modifications such as cytosine methylation is also informative, considering its roles in gene expression regulation. In our previous study, differentially transcribed fragments (DTFs) were obtained using cDNA-amplified fragment length polymorphism by comparing mock- and SCMV-inoculated plants from two sugarcane cultivars with contrasting responses to SMD. In this study, the identification of unexplored DTFs was continued while the same leaf samples were used to evaluate SCMV-mediated changes in the cytosine methylation pattern by using methylation-sensitive amplification polymorphism. This analysis revealed minor changes in cytosine methylation in response to SCMV infection, but distinct changes between the cultivars with contrasting responses to SMD, with higher hypomethylation events 24 and 72 h post-inoculation in the resistant cultivar. The differentially methylated fragments (DMFs) aligned with transcripts, putative promoters, and genomic regions, with a preponderant distribution within CpG islands. The transcripts found were associated with plant immunity and other stress responses, epigenetic changes, and transposable elements. The DTFs aligned with transcripts assigned to stress responses, epigenetic changes, photosynthesis, lipid transport, and oxidoreductases, in which the transcriptional start site is located in proximity with CpG islands and tandem repeats. Real-time quantitative polymerase chain reaction results revealed significant upregulation in the resistant cultivar of aspartyl protease and VQ protein, respectively, selected from DMF and DTF alignments, suggesting their roles in genetic resistance to SMD and supporting the influence of cytosine methylation in gene expression. Thus, we identified new candidate genes for further validation and showed that the changes in cytosine methylation may regulate important mechanisms underlying the genetic resistance to SMD.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias Agr & Vet FCAV, Biol Aplicada Agr, Jaboticabal, SP, Brazil-
Descrição: dc.descriptionInst Biol, Crop Protect Res Ctr, Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Fed Sao Paulo, Dept Ciencia & Tecnol, Inst Ciencia & Tecnol, Sao Jose Dos Campos, SP, Brazil-
Descrição: dc.descriptionInst Agron, Ctr Cana, Ribeirao Preto, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias Agr & Vet FCAV, Biol Aplicada Agr, Jaboticabal, SP, Brazil-
Descrição: dc.descriptionCNPq: 424050/2016-1-
Descrição: dc.descriptionFAPESP: 2012/15060-6-
Descrição: dc.descriptionFAPESP: 08/56146-5-
Descrição: dc.descriptionCAPES: 001-
Formato: dc.format24-
Idioma: dc.languageen-
Publicador: dc.publisherPublic Library Science-
Relação: dc.relationPlos One-
???dc.source???: dc.sourceWeb of Science-
Título: dc.titleSugarcane mosaic virus mediated changes in cytosine methylation pattern and differentially transcribed fragments in resistance-contrasting sugarcane genotypes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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