Generation of neural progenitor cells from porcine-induced pluripotent stem cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Copenhagen-
Autor(es): dc.creatorMachado, Lucas Simoes-
Autor(es): dc.creatorPieri, Naira Caroline Godoy-
Autor(es): dc.creatorBotigelli, Ramon Cesar [UNESP]-
Autor(es): dc.creatorCastro, Raquel Vasconcelos Guimaraes de [UNESP]-
Autor(es): dc.creatorSouza, Aline Fernanda de-
Autor(es): dc.creatorBridi, Alessandra-
Autor(es): dc.creatorLima, Marina Amaro-
Autor(es): dc.creatorFantinato Neto, Paulo-
Autor(es): dc.creatorPessoa, Lais Vicari de Figueiredo-
Autor(es): dc.creatorMartins, Simone Maria Massami Kitamura-
Autor(es): dc.creatorDe Andrade, Andre Furugen Cesar-
Autor(es): dc.creatorFreude, Kristine Karla-
Autor(es): dc.creatorBressan, Fabiana Fernandes-
Data de aceite: dc.date.accessioned2022-02-22T00:57:20Z-
Data de disponibilização: dc.date.available2022-02-22T00:57:20Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-22-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/term.3143-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209677-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209677-
Descrição: dc.descriptionIn this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages, and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, beta-TUBULIN III, and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research.-
Descrição: dc.descriptionCoordination for the Improvement of Higher Education Personnel-
Descrição: dc.descriptionNational Council for Scientific and Technological Development-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Postgrad Program Anat Domest & Wild Anim, Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Anim Sci, Pirassununga, Brazil-
Descrição: dc.descriptionUniv Copenhagen, Dept Vet & Anim Sci, Copenhagen, Denmark-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, Brazil-
Descrição: dc.descriptionNational Council for Scientific and Technological Development: 433133/2018-0-
Descrição: dc.descriptionFAPESP: 2013/08135-2-
Descrição: dc.descriptionFAPESP: 2015/26818-5-
Descrição: dc.descriptionFAPESP: 2017/02159-8-
Descrição: dc.descriptionFAPESP: 2017/13973-8-
Formato: dc.format1880-1891-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-Blackwell-
Relação: dc.relationJournal Of Tissue Engineering And Regenerative Medicine-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectcellular reprogramming-
Palavras-chave: dc.subjectinduced pluripotent stem cells (iPSC)-
Palavras-chave: dc.subjectneural progenitor cells-
Palavras-chave: dc.subjectswine-
Título: dc.titleGeneration of neural progenitor cells from porcine-induced pluripotent stem cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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