Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorEzequiel Dias Fdn-
Autor(es): dc.contributorGrp Oncoclin-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorGoncalves, Bryan Ortero Perez-
Autor(es): dc.creatorDe Andrade, Warne Pedro [UNESP]-
Autor(es): dc.creatorDa Conceicao Braga, Leticia-
Autor(es): dc.creatorFialho, Silvia Ligorio-
Autor(es): dc.creatorSilva, Luciana Maria-
Data de aceite: dc.date.accessioned2022-02-22T00:57:16Z-
Data de disponibilização: dc.date.available2022-02-22T00:57:16Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3892/ol.2020.12019-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209666-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209666-
Descrição: dc.descriptionThe epithelial-to-mesenchymal transition (EMT) is a phenomenon during which cancer epithelial cells undergo changes in plasticity and lose cell-cell adhesion with consequent remodeling of the extracellular matrix and development of mesenchymal characteristics. Long non-coding RNAs (lncRNAs) have been described as EMT modulation markers, becoming a promising target in the development of new therapies for cancer. The present study aimed to investigate the role of everolimus at 100 nM as inductor of the EMT phenomenon in cell lines derived from human breast (BT-549), colorectal (RKO-AS45-1) and ovary (TOV-21G) cancer. The integrity of cellular junctions was monitored using an in vitro model of epithelial resistance. The results demonstrated that the EMT genes ZEB1, TWIST1 and TGFB1 were differentially expressed in cells treated with everolimus compared with in untreated cells. lncRNA HOTAIR was upregulated post-treatment only in BT-549 cells compared with in untreated cells. After treatment with everolimus, the intensity of fluorescence of P-cadherin decreased, and that of fibronectin increased in RKO-AS45-1 and TOV-21G cells compared with control cells. The transepithelial electrical resistance at the RKO-AS45-1 monolayer treated with everolimus started to decrease at 48 h. The changes in the gene expression and epithelial resistance may confirm the role of everolimus in EMT.-
Descrição: dc.descriptionEzequiel Dias Fdn, Cellular Biol Res & Dev Dept, BR-30510010 Belo Horizonte, MG, Brazil-
Descrição: dc.descriptionGrp Oncoclin, Hematol & Oncol Nucleus, BR-30140001 Belo Horizonte, MG, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Sch Med, Dept Obstet & Gynecol, BR-18618687 Botucatu, SP, Brazil-
Descrição: dc.descriptionEzequiel Dias Fdn, Pharmaceut Res & Dev, BR-30510010 Belo Horizonte, MG, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Sch Med, Dept Obstet & Gynecol, BR-18618687 Botucatu, SP, Brazil-
Formato: dc.format11-
Idioma: dc.languageen-
Publicador: dc.publisherSpandidos Publ Ltd-
Relação: dc.relationOncology Letters-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectEMT-
Palavras-chave: dc.subjectcancer-
Palavras-chave: dc.subjecteverolimus-
Palavras-chave: dc.subjectHOTAIR-
Palavras-chave: dc.subjectbiomarker-
Título: dc.titleEpithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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