Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorOakland Univ Math & Sci-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorCamargo Reis Mello, Daphne de [UNESP]-
Autor(es): dc.creatorRodrigues, Lais Morandini [UNESP]-
Autor(es): dc.creatorD'Antola Mello, Fabia Zampieri [UNESP]-
Autor(es): dc.creatorGoncalves, Thais Fernanda [UNESP]-
Autor(es): dc.creatorFerreira, Bento-
Autor(es): dc.creatorSchneider, Sandra Giacomin-
Autor(es): dc.creatorOliveira, Luciane Dias de [UNESP]-
Autor(es): dc.creatorVasconcellos, Luana Marotta Reis de [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:57:02Z-
Data de disponibilização: dc.date.available2022-02-22T00:57:02Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-10-24-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s40729-020-00261-3-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209590-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209590-
Descrição: dc.descriptionBackground An effective biomaterial for bone replacement should have properties to avoid bacterial contamination and promote bone formation while inducing rapid cell differentiation simultaneously. Bone marrow stem cells are currently being investigated because of their known potential for differentiation in osteoblast lineage. This makes these cells a good option for stem cell-based therapy. We have aimed to analyze, in vitro, the potential of pure titanium (Ti), Ti-35Nb-7Zr alloy (A), niobium (Nb), and zirconia (Zr) to avoid the microorganisms S. aureus (S.a) and P. aeruginosa (P.a). Furthermore, our objective was to evaluate if the basic elements of Ti-35Nb-7Zr alloy have any influence on bone marrow stromal cells, the source of stem cells, and observe if these metals have properties to induce cell differentiation into osteoblasts. Methods Bone marrow stromal cells (BMSC) were obtained from mice femurs and cultured in osteogenic media without dexamethasone as an external source of cell differentiation. The samples were divided into Ti-35Nb-7Zr alloy (A), pure titanium (Ti), Nb (niobium), and Zr (zirconia) and were characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). After predetermined periods, cell interaction, cytotoxicity, proliferation, and cell differentiation tests were performed. For monotypic biofilm formation, standardized suspensions (10(6) cells/ml) with the microorganisms S. aureus (S.a) and P. aeruginosa (P.a) were cultured for 24 h on the samples and submitted to an MTT test. Results All samples presented cell proliferation, growth, and spreading. All groups presented cell viability above 70%, but the alloy (A) showed better results, with statistical differences from Nb and Zr samples. Zr expressed higher ALP activity and was statistically different from the other groups (p < 0.05). In contrast, no statistical difference was observed between the samples as regards mineralization nodules. Lower biofilm formation of S.a and P.a. was observed on the Nb samples, with statistical differences from the other samples. Conclusion Our results suggest that the basic elements present in the alloy have osteoinductive characteristics, and Zr has a good influence on bone marrow stromal cell differentiation. We also believe that Nb has the best potential for reducing the formation of microbial biofilms.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniv Estadual Paulista UNESP, Sao Jose Dos Campos Sch Dent, Dept Biosci & Oral Diag, Av Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP, Brazil-
Descrição: dc.descriptionOakland Univ Math & Sci, 318 Meadow Brook Rd, Rochester Hills, MI 48309 USA-
Descrição: dc.descriptionEscola Engn Lorena EEL USP, Gleba Al-6 S-N, Lorena, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista UNESP, Sao Jose Dos Campos Sch Dent, Dept Biosci & Oral Diag, Av Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP, Brazil-
Formato: dc.format11-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationInternational Journal Of Implant Dentistry-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectTi-35Nb-7Zr alloy-
Palavras-chave: dc.subjectBiofilm-
Palavras-chave: dc.subjectBone marrow cells-
Palavras-chave: dc.subjectOsteogenesis-
Título: dc.titleBiological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering-
Tipo de arquivo: dc.typelivro digital-
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