Anti-Acanthamoeba synergistic effect of chlorhexidine and Garcinia mangostana extract or alpha-mangostin against Acanthamoeba triangularis trophozoite and cyst forms

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Autor(es): dc.contributorWalailak Univ-
Autor(es): dc.contributorPrince Songkla Univ-
Autor(es): dc.contributorChulalongkorn Univ-
Autor(es): dc.contributorIslamic Univ Uganda-
Autor(es): dc.contributorMahidol Univ-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorNatl Inst TB & Resp Dis NITRD-
Autor(es): dc.contributorAveiro Inst Mat-
Autor(es): dc.contributorUniv Aveiro-
Autor(es): dc.contributorUniv Dev Alternat Lalmatia-
Autor(es): dc.contributorSch Pharm-
Autor(es): dc.contributorBirjand Univ Med Sci-
Autor(es): dc.contributorUniv Tabriz-
Autor(es): dc.contributorUNSW-
Autor(es): dc.contributorCtr Biomed Res-
Autor(es): dc.creatorSangkanu, Suthinee-
Autor(es): dc.creatorMitsuwan, Watcharapong-
Autor(es): dc.creatorMahabusarakam, Wilawan-
Autor(es): dc.creatorJimoh, Tajudeen O.-
Autor(es): dc.creatorWilairatana, Polrat-
Autor(es): dc.creatorGirol, Ana Paula [UNESP]-
Autor(es): dc.creatorVerma, Ajoy K.-
Autor(es): dc.creatorPereira, Maria de Lourdes-
Autor(es): dc.creatorRahmatullah, Mohammed-
Autor(es): dc.creatorWiart, Christophe-
Autor(es): dc.creatorSiyadatpanah, Abolghasem-
Autor(es): dc.creatorNorouzi, Roghayeh-
Autor(es): dc.creatorMutombo, Polydor Ngoy-
Autor(es): dc.creatorNissapatorn, Veeranoot-
Data de aceite: dc.date.accessioned2022-02-22T00:56:02Z-
Data de disponibilização: dc.date.available2022-02-22T00:56:02Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-04-13-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-021-87381-x-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209330-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209330-
Descrição: dc.descriptionAcanthamoeba spp. can cause amoebic keratitis (AK). Chlorhexidine is effective for AK treatment as monotherapy, but with a relative failure on drug bioavailability in the deep corneal stroma. The combination of chlorhexidine and propamidine isethionate is recommended in the current AK treatment. However, the effectiveness of treatment depends on the parasite and virulence strains. This study aims to determine the potential of Garcinia mangostana pericarp extract and alpha -mangostin against Acanthamoeba triangularis, as well as the combination with chlorhexidine in the treatment of Acanthamoeba infection. The minimal inhibitory concentrations (MICs) of the extract and alpha -mangostin were assessed in trophozoites with 0.25 and 0.5 mg/mL, for cysts with 4 and 1 mg/mL, respectively. The MIC of the extract and alpha -mangostin inhibited the growth of A. triangularis trophozoites and cysts for up to 72 h. The extract and alpha -mangostin combined with chlorhexidine demonstrated good synergism, resulting in a reduction of 1/4-1/16 of the MIC. The SEM results showed that Acanthamoeba cells treated with a single drug and its combination caused damage to the cell membrane and irregular cell shapes. A good combination displayed by the extract or alpha -mangostin and chlorhexidine, described for the first time. Therefore, this approach is promising as an alternative method for the management of Acanthamoeba infection in the future.-
Descrição: dc.descriptionRoyal Patronage of Her Royal Highness Princess Maha Chakri Sirindhorn-Botanical Garden of Walailak University, Nakhon Si Thammarat-
Descrição: dc.descriptionproject CICECO-Aveiro Institute of Materials-
Descrição: dc.descriptionnational funds by FCT/MCTES-
Descrição: dc.descriptionWalailak Univ, Sch Allied Hlth Sci, World Union Herbal Drug Discovery WUHeDD, Southeast Asia Water Team,SEA Water Team, Nakhon Si Thammarat, Thailand-
Descrição: dc.descriptionWalailak Univ, Res Excellence Ctr Innovat & Hlth Prod RECIHP, Nakhon Si Thammarat, Thailand-
Descrição: dc.descriptionWalailak Univ, Akkhraratchakumari Vet Coll, Nakhon Si Thammarat, Thailand-
Descrição: dc.descriptionWalailak Univ, Res Ctr Excellence Innovat Essential Oil, Nakhon Si Thammarat, Thailand-
Descrição: dc.descriptionPrince Songkla Univ, Fac Sci, Div Phys Sci, Hat Yai, Thailand-
Descrição: dc.descriptionChulalongkorn Univ, Fac Pharmaceut Sci, Dept Pharmacognosy & Pharmaceut Bot, Bangkok, Thailand-
Descrição: dc.descriptionIslamic Univ Uganda, Habib Med Sch, Dept Biochem, Kampala, Uganda-
Descrição: dc.descriptionMahidol Univ, Fac Trop Med, Dept Clin Trop Med, Bangkok, Thailand-
Descrição: dc.descriptionSao Paulo State Univ, Fac Sci, Dept Biol, Sao Paulo, Brazil-
Descrição: dc.descriptionNatl Inst TB & Resp Dis NITRD, Dept Microbiol, New Delhi, India-
Descrição: dc.descriptionAveiro Inst Mat, Dept Med Sci, CICECO, Aveiro, Portugal-
Descrição: dc.descriptionUniv Aveiro, Aveiro, Portugal-
Descrição: dc.descriptionUniv Dev Alternat Lalmatia, Dept Biotechnol & Genet Engn, Dhaka, Bangladesh-
Descrição: dc.descriptionSch Pharm, Univ Nottingham Malaysia Campus, Semenyih, Selangor, Malaysia-
Descrição: dc.descriptionBirjand Univ Med Sci, Ferdows Sch Paramed & Hlth, Birjand, Iran-
Descrição: dc.descriptionUniv Tabriz, Fac Vet Med, Dept Pathobiol, Tabriz, Iran-
Descrição: dc.descriptionUNSW, Sch Publ Hlth & Community Med, UNSW Med, Sydney, NSW, Australia-
Descrição: dc.descriptionCtr Biomed Res, Burnet Inst, Melbourne, Vic, Australia-
Descrição: dc.descriptionSao Paulo State Univ, Fac Sci, Dept Biol, Sao Paulo, Brazil-
Descrição: dc.descriptionRoyal Patronage of Her Royal Highness Princess Maha Chakri Sirindhorn-Botanical Garden of Walailak University, Nakhon Si Thammarat: WUBG020-2564-
Descrição: dc.descriptionproject CICECO-Aveiro Institute of Materials: UIDB/50011/2020-
Descrição: dc.descriptionproject CICECO-Aveiro Institute of Materials: UIDP/50011/2020-
Formato: dc.format11-
Idioma: dc.languageen-
Publicador: dc.publisherNature Research-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceWeb of Science-
Título: dc.titleAnti-Acanthamoeba synergistic effect of chlorhexidine and Garcinia mangostana extract or alpha-mangostin against Acanthamoeba triangularis trophozoite and cyst forms-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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