NonSurgical Embryo Recovery from Estrus-Synchronized or Superovulated Morada Nova Ewes: A Feasible Strategy for Sheep Embryo Banking

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniv Fed Rural Rio De Janeiro-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal Fluminense (UFF)-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorUniv Guelph-
Autor(es): dc.creatorArrais, Aline Matos-
Autor(es): dc.creatorMello, Marco Roberto Bourg de-
Autor(es): dc.creatorVergani, Gabriel Brun [UNESP]-
Autor(es): dc.creatorFigueira, Lucas Machado-
Autor(es): dc.creatorEsteves, Sergio Novita-
Autor(es): dc.creatorPereira, Veronica Schinaider do Amaral-
Autor(es): dc.creatorBartlewski, Pawel Mieczyslaw-
Autor(es): dc.creatorOliveira, Maria Emilia Franco [UNESP]-
Autor(es): dc.creatorSouza-Fabjan, Joanna Maria Goncalves-
Autor(es): dc.creatorFonseca, Jeferson Ferreira da-
Data de aceite: dc.date.accessioned2022-02-22T00:55:56Z-
Data de disponibilização: dc.date.available2022-02-22T00:55:56Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-03-25-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1089/bio.2020.0125-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209291-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209291-
Descrição: dc.descriptionThis study assessed the feasibility of in vivo embryo production and nonsurgical embryo recovery (NSER) in Morada Nova ewes (an endangered native Brazilian breed of sheep) subjected to different estrus synchronization and/or superovulation protocols. Ewes received intravaginal sponges soaked with 60 mg medroxyprogesterone acetate (MAP), which were kept in place for six (G6; n = 12), nine (G9; n = 12), or 12 (G12; n = 12) days. Half of the ewes in each group remained estrus synchronized only ((SYNCH)) and the other half was superovulated ((SOV)) with 133 mg porcine follicle-stimulating hormone (pFSH). There were no differences (p > 0.05) in antral follicle counts determined with ultrasonography 60 hours before MAP sponge removal (or at the time of the first pFSH dose) among G6 (6.4 +/- 0.9), G9 (6.2 +/- 0.7), and G12 (5.5 +/- 0.6). Estrus responses and NSER success rates did not vary (p > 0.05) among the three progestin-treatment groups of ewes for either estrus-induced or superovulated animals. The onset of estrus occurred 10-12 hours later (p < 0.01) in G9(SYNCH) ewes compared with G6(SYNCH) and G12(SYNCH,) and the duration of estrus was similar to 19 hours greater (p < 0.01) in G9(SOV) than in G6(SOV). The average duration of the NSER procedure was 32.6 +/- 1.3 minutes. At least one structure was recovered in 85.7% of synchronized and in 87.5% of superovulated ewes. Viable embryo recovery rates were also similar (p > 0.05) for G6 (1.0 +/- 0.3 and 2.5 +/- 1.5), G9 (1.3 +/- 0.5 and 4.8 +/- 2.0), and G12 groups (1.0 +/- 0.3 and 4.8 +/- 2.3; estrus-synchronized and superovulated ewes, respectively). In conclusion, progestogen pretreatment of different durations and NSER can be employed in Morada Nova ewes, resulting in reasonable viable embryo recovery rates in both estrus-synchronized and superovulated animals. Therefore, both techniques are suitable for use in commercial settings as well as small ruminant conservation programs.-
Descrição: dc.descriptionEmbrapa Goats and Sheep-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniv Fed Rural Rio De Janeiro, Dept Reprod & Avaliacao Anim, Seropedica, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, Brazil-
Descrição: dc.descriptionUniv Fed Fluminense, Fac Vet, Niteroi, RJ, Brazil-
Descrição: dc.descriptionEmbrapa Pecuaria Sudeste, Sao Carlos, Brazil-
Descrição: dc.descriptionUniv Guelph, Dept Biomed Sci, Ontario Vet Coll, Guelph, ON, Canada-
Descrição: dc.descriptionEmbrapa Caprinos & Ovinos, Rodovia MG 133,Km 42, BR-36155000 Coronel Pacheco, MG, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, Brazil-
Descrição: dc.descriptionEmbrapa Goats and Sheep: Superovi-22.13.06.026.00.03-
Descrição: dc.descriptionEmbrapa Goats and Sheep: 22.13.06.026.00.04-
Descrição: dc.descriptionFAPEMIG: CVZ-PPM 00201-17-
Descrição: dc.descriptionCAPES: 001-
Formato: dc.format9-
Idioma: dc.languageen-
Publicador: dc.publisherMary Ann Liebert, Inc-
Relação: dc.relationBiopreservation And Biobanking-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectin vivo embryo production-
Palavras-chave: dc.subjectnaturalized breed-
Palavras-chave: dc.subjectNSER-
Palavras-chave: dc.subjectovine-
Palavras-chave: dc.subjectsuperovulation-
Título: dc.titleNonSurgical Embryo Recovery from Estrus-Synchronized or Superovulated Morada Nova Ewes: A Feasible Strategy for Sheep Embryo Banking-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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