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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Univ Minnesota | - |
Autor(es): dc.contributor | Univ Saskatchewan | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Univ Utrecht | - |
Autor(es): dc.creator | Leis, Marina L. | - |
Autor(es): dc.creator | Madruga, Gabriela M. [UNESP] | - |
Autor(es): dc.creator | Costa, Matheus O. | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:55:43Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:55:43Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-02-18 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1371/journal.pone.0247392 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/209224 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/209224 | - |
Descrição: dc.description | Purpose The ocular surface microbiome has been described as paucibacterial. Until now, studies investigating the bacterial community associated with the ocular surface through high-throughput sequencing have focused on the conjunctiva. Conjunctival samples are thought to reflect and be representative of the microbiome residing on the ocular surface, including the cornea. Here, we hypothesized that the bacterial community associated with the corneal surface was different from those of the inferonasal and superotemporal conjunctival fornices, and from the tear film. Methods Both eyes from 15 healthy piglets were sampled using swabs (inferonasal fornix, superotemporal fornix, and corneal surface, n = 30 each) and Schirmer tear test strips (STT, n = 30). Negative sampling controls (swabs and STT, n = 2 each) and extraction controls (n = 4) were included. Total DNA was extracted and high-throughput sequencing targeting the 16S rRNA gene was performed. Bioinformatic analyses included multiple contamination-controlling steps. Results Corneal surface samples had a significantly lower number of taxa detected (P<0.01) and were compositionally different from all other sample types (Bray-Curtis dissimilarity, P<0.04). It also harbored higher levels of Proteobacteria (P<0.05), specifically Brevundimonas spp. (4.1-fold) and Paracoccus spp. (3.4-fold) than other sample types. Negative control STT strip samples yielded the highest amount of 16S rRNA gene copies across all sample types (P<0.05). Conclusions Our data suggests that the corneal surface provides a distinct environmental niche within the ocular surface, leading to a bacterial community compositionally different from all other sample types. | - |
Descrição: dc.description | department of Veterinary Clinical Sciences (VCS) at the University of Minnesota (UMN) | - |
Descrição: dc.description | Univ Minnesota, Coll Vet Med, Vet Clin Sci, Minneapolis, MN 55455 USA | - |
Descrição: dc.description | Univ Saskatchewan, Western Coll Vet Med, Small Anim Clin Sci, Saskatoon, SK, Canada | - |
Descrição: dc.description | State Univ Sao Paulo, Sao Paulo, Brazil | - |
Descrição: dc.description | Univ Saskatchewan, Western Coll Vet Med, Large Anim Clin Sci, Saskatoon, SK, Canada | - |
Descrição: dc.description | Univ Utrecht, Fac Vet Med, Dept Populat Hlth, Utrecht, Netherlands | - |
Descrição: dc.description | State Univ Sao Paulo, Sao Paulo, Brazil | - |
Formato: dc.format | 16 | - |
Idioma: dc.language | en | - |
Publicador: dc.publisher | Public Library Science | - |
Relação: dc.relation | Plos One | - |
???dc.source???: dc.source | Web of Science | - |
Título: dc.title | The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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