Extraction of Bioactive Proteins from Seeds (Corn, Sorghum, and Sunflower) and Sunflower Byproduct: Enzymatic Hydrolysis and Antioxidant Properties

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorGoiano Fed Inst Educ Sci & Technol-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal do Rio de Janeiro (UFRJ)-
Autor(es): dc.creatorPrado, Danielle M. F. do-
Autor(es): dc.creatorAlmeida, Adrielle B. de-
Autor(es): dc.creatorOliveira Filho, Josemar G. de [UNESP]-
Autor(es): dc.creatorAlves, Cassia C. F.-
Autor(es): dc.creatorEgea, Mariana B.-
Autor(es): dc.creatorLemes, Ailton C.-
Data de aceite: dc.date.accessioned2022-02-22T00:55:39Z-
Data de disponibilização: dc.date.available2022-02-22T00:55:39Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.2174/1573401316999200731005803-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209198-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209198-
Descrição: dc.descriptionBackground: Food proteins have benefits for human health, which justifies their production and use. In this context, the use of seeds and byproducts that would be otherwise discarded is highlighted in the present work, to produce protein extracts and hydrolyzed proteins, generating opportunities to reduce environmental impacts. Objective: This work aimed to use different extraction methods to obtain protein extracts from seeds (corn, sorghum, and sunflower) and sunflower byproduct to determine their antioxidant activity, and apply different proteolytic enzymes in the hydrolysis of sunflower byproduct. Methods: The seeds of corn, sorghum, and sunflower, and sunflower byproduct were ground to produce flour and the protein extracts were prepared using five different methods. The bioactivity of fractions was analyzed by different methods (ABTS, DPPH, and FRAP) to evaluate the antioxidant activity. Results: The most effective methods, which resulted in higher protein extraction and antioxidant activity, were those in which NH4HCO3 (5 mM, pH 8.0) and H2O/C2H6O (2:3) were used. The highest protein contents were 797.9, 303.8, and 11296.5 mu g/g, and the highest antioxidant activity was 34417.5, 9732.6, and 47473.1 mu g TE/g from Soxhlet and Bligh and Dyer defatted extractions for sunflower seed, and sunflower byproduct, respectively. Regarding enzymatic hydrolysis, sunflower byproduct was the substrate that presented the highest degree of hydrolysis (11.06%) when Neutrase (R) enzyme was used. Enzymatic hydrolysis increased antioxidant activity in the hydrolyzed proteins, approximately by 20.0%, using Neutrase (R) Neutrase and 22.3% using Flavourzyme (R) Flavourzyme treatments. Conclusion: The protein extracts and the hydrolyzed proteins exhibited high antioxidant activity, demonstrating great potential for use as natural antioxidants in food systems.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundacao de Amparo a Pesquisa do Estado de Goias (FAPEG)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionInstituto Federal Goiano (IFGoiano)-
Descrição: dc.descriptionUniversidade Federal do Rio de Janeiro (UFRJ) -Brazil-
Descrição: dc.descriptionGoiano Fed Inst Educ Sci & Technol, Campus Rio Verde,Rodovia Sul Goiana,Km 01, BR-75901970 Rio Verde, Go, Brazil-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Sch Pharmaceut Sci, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP, Brazil-
Descrição: dc.descriptionFed Univ Rio de Janeiro UFRJ, Sch Chem, Dept Biochem Engn, Athos da Silveira Ramos 149, BR-21941909 Rio De Janeiro, Brazil-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Sch Pharmaceut Sci, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP, Brazil-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionInstituto Federal Goiano (IFGoiano): 23218.001954/2019-16-
Formato: dc.format310-320-
Idioma: dc.languageen-
Publicador: dc.publisherBentham Science Publ Ltd-
Relação: dc.relationCurrent Nutrition & Food Science-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectExtraction methods-
Palavras-chave: dc.subjectprotein extraction-
Palavras-chave: dc.subjecthydrolysis-
Palavras-chave: dc.subjectproteolytic enzymes-
Palavras-chave: dc.subjectbyproducts-
Palavras-chave: dc.subjectnatural antioxidants-
Título: dc.titleExtraction of Bioactive Proteins from Seeds (Corn, Sorghum, and Sunflower) and Sunflower Byproduct: Enzymatic Hydrolysis and Antioxidant Properties-
Tipo de arquivo: dc.typelivro digital-
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