Polyaniline-based field effect transistor for DNA/RNA biomarker sensing: Comparison to electrochemical impedance and inorganic layer

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorPedroza Dias Mello, Hugo Jose Nogueira-
Autor(es): dc.creatorBachour Junior, Bassam-
Autor(es): dc.creatorMulato, Marcelo-
Data de aceite: dc.date.accessioned2022-02-22T00:55:27Z-
Data de disponibilização: dc.date.available2022-02-22T00:55:27Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.sna.2020.112481-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/209120-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/209120-
Descrição: dc.descriptionOne relevant aspect of the research in materials science focus on studying materials properties for applications in the field of sensing and biosensing devices aiming to obtain portable, stable, sensitive, and low-cost detection systems for a broad range of diseases, traumas, and infections. We report a polyaniline (PANI)-based extended-gate field effect transistor DNA/RNA (DNA-EGFET) biosensor. The biosensor is based on a functionalized Au reference electrode with thiolated bio-receptor single-strand DNA (ssDNA) corresponding to the mu DNA-224, an overexpressed DNA biomarker sample for rectal colon cancer, and the transduction system is based on an electrodeposited PANI thin film as sensing stage. Variations on net surface charge of the hybridized single strand DNA led to changes of the output voltage from the FET system, which could be correlated to the concentration of the detected complementary DNA. Comparison between the potentiometric EGFET biosensor and the electrochemical impedance spectroscopy (EIS) biosensor used for DNA/RNA measurements and the use of organic and inorganic (metal oxide) semiconducting sensing materials were performed. This DNA-EGFET biosensor offered fast response, sensitivity of 3.3 +/- 0.3 mV/log[tDNA], linearity of 96 % in a detection range from 1 pmol/L to 1 mu mol/L and LoD of 9.77 pmol/L in buffer solution. The application and comparison of the PANI based DNA-EGFET for measurements of DNA biomarkers was demonstrated as a proof-of-concept of the advantages and easy fabrication of the device. (C) 2020 Elsevier B.V. All rights reserved.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniv Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Phys, BR-14040901 Ribeirao Preto, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2017/24201-6-
Descrição: dc.descriptionFAPESP: 2014/24559-0-
Descrição: dc.descriptionCNPq: 308713/2018-4-
Formato: dc.format7-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationSensors And Actuators A-physical-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectField-effect transistor-
Palavras-chave: dc.subjectImpedimetric-
Palavras-chave: dc.subjectSemiconducting polymer-
Palavras-chave: dc.subjectBiosensor-
Palavras-chave: dc.subjectDNA-
Título: dc.titlePolyaniline-based field effect transistor for DNA/RNA biomarker sensing: Comparison to electrochemical impedance and inorganic layer-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.