Genome-wide profiling and predicted significance of post-mortem brain microRNA in Alzheimer's disease

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFed Univ Brasilia UnB-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorMcGill Univ Hlth Ctr MUHC-
Autor(es): dc.creatorHenriques, Adriane D.-
Autor(es): dc.creatorMachado-Silva, Wilcelly-
Autor(es): dc.creatorLeite, Renata E. P. [UNESP]-
Autor(es): dc.creatorSuemoto, Claudia K. [UNESP]-
Autor(es): dc.creatorLeite, Katia R. M. [UNESP]-
Autor(es): dc.creatorSrougi, Miguel [UNESP]-
Autor(es): dc.creatorPereira, Alexandre C. [UNESP]-
Autor(es): dc.creatorJacob-Filho, Wilson [UNESP]-
Autor(es): dc.creatorNobrega, Otavio T.-
Autor(es): dc.creatorBrazilian Aging Brain Study Grp-
Data de aceite: dc.date.accessioned2022-02-22T00:54:39Z-
Data de disponibilização: dc.date.available2022-02-22T00:54:39Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.mad.2020.111352-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208875-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208875-
Descrição: dc.descriptionBackground: MicroRNAs (miRNAs) emerged as regulatory elements, with up to 70 % of all miRNAs found in the brain, playing key roles in the onset of Alzheimer's disease (AD). Objective: to broadly assess the expression levels of miRNAs in post-mortem brain (PMB) samples of individuals deceased with or without AD. Methods: A high-throughput micmarray platform was used to sketch miRNA samples isolated from superior and middle temporal gyrus of A+T+ AD cases, compared to samples from age- and sex-matched AD-devoid donors, all pulled from the University of Sao Paulo's Brain Biobank. The miRNAs identified by microarray were subjected to validation with specific qRT-PCR assays employing independent PMB samples. Results: The analyses yielded 6 miRNAs differentially expressed (miR-30e_3p; miR-365b_5p; miR-664_3p; miR1202; miR-4286; miR-4449), and their interplay with specific AD-related genes and signaling pathways was explored using bioinformatics analyses (including the KEGG package, mirPath v.3). In the end, 3 miRNAs, 7 target genes and 11 pathways were found closely interrelated and implicated with the AD pathophysiology. Conclusion: A dysregulation on a subset of these miRNAs appear to affect a range of genes (notably PTEN) and pathways (emphasis to PI3K-AKT) so to provide grounds for neuronal death by apoptotic signaling, autophagy and/or oxidative damage.-
Descrição: dc.descriptionFundacao de Amparo a Pesquisa do Distrito Federal (FAP-DF)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFed Univ Brasilia UnB, Brasilia, DF, Brazil-
Descrição: dc.descriptionState Univ Sao Paulo USP, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionMcGill Univ Hlth Ctr MUHC, Montreal, PQ, Canada-
Descrição: dc.descriptionState Univ Sao Paulo USP, Sao Paulo, SP, Brazil-
Descrição: dc.descriptionFundacao de Amparo a Pesquisa do Distrito Federal (FAP-DF): 193.000.967/2015-
Descrição: dc.descriptionCNPq: 445692/2014-6-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionCNPq: 303,540/2019 2-
Formato: dc.format9-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationMechanisms Of Ageing And Development-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectAlzheimer's disease-
Palavras-chave: dc.subjectmicroRNA-
Palavras-chave: dc.subjectArray analysis-
Palavras-chave: dc.subjectDIANA miRPath-
Título: dc.titleGenome-wide profiling and predicted significance of post-mortem brain microRNA in Alzheimer's disease-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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