Molecular-level effects on cell membrane models to explain the phototoxicity of gold shell-isolated nanoparticles to cancer cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorCamacho, Sabrina A. [UNESP]-
Autor(es): dc.creatorKobal, Mirella B. [UNESP]-
Autor(es): dc.creatorAlmeida Jr, Alexandre M. [UNESP]-
Autor(es): dc.creatorToledo, Karina A. [UNESP]-
Autor(es): dc.creatorOliveira Jr, Osvaldo N.-
Autor(es): dc.creatorAoki, Pedro H. B. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:54:25Z-
Data de disponibilização: dc.date.available2022-02-22T00:54:25Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.colsurfb.2020.111189-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208787-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208787-
Descrição: dc.descriptionMetallic nanoparticles are promising agents for photothermal cancer therapy (PTT) owing to their photostability and efficient light-to-heat conversion, but their possible aggregation remains an issue. In this paper, we report on the photoinduced heating of gold shell-isolated nanoparticles (AuSHINs) in in vitro experiments to kill human oropharyngeal (HEp-2) and breast (BT-474 and MCF-7) carcinoma cells, with cell viability reducing below 50 % with 2.2 x 10(12) AuSHINs/mL and 6 h of incubation. This toxicity to cancer cells is significantly higher than in previous works with gold nanoparticles. Considering the AuSHINs dimensions we hypothesize that cell uptake is not straightforward, and the mechanism of action involves accumulation on phospholipid membranes as the PTT target for photoinduced heating and subsequent generation of reactive oxygen species (ROS). Using Langmuir monolayers as simplified membrane models, we confirmed that AuSHINs have a larger effect on 1,2-dioleoyl-snglycero-3-phospho-L-serine (DOPS), believed to represent cancer cell membranes, than on 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) taken as representative of healthy eukaryotic cells. In particular, data from polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) revealed an increased conformational order of DOPS tails due to the stronger adsorption of AuSHINs. Furthermore, light irradiation reduced the stability of AuSHINs containing DOPC and DOPS monolayers owing to oxidative reactions triggered by ROS upon photoinduced heating. Compared to DOPC, DOPS lost nearly twice as much material to the subphase, which is consistent with a higher rate of ROS formation in the vicinity of the DOPS monolayer.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionINEO-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Sch Sci Humanities & Languages, BR-19806900 Assis, SP, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Sao Carlos Inst Phys, CP 369, BR-13566590 Sao Carlos, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Sch Sci Humanities & Languages, BR-19806900 Assis, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2018/14692-5-
Descrição: dc.descriptionFAPESP: 2018/16713-0-
Descrição: dc.descriptionFAPESP: 2013/14262-7-
Descrição: dc.descriptionFAPESP: 2018/22214-6-
Descrição: dc.descriptionCNPq: Universal 403713/2016-1-
Formato: dc.format10-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationColloids And Surfaces B-biointerfaces-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectPhotothermal cancer therapy (PTT)-
Palavras-chave: dc.subjectGold shell-isolated nanoparticles (AuSHINs)-
Palavras-chave: dc.subjectLangmuir monolayers-
Título: dc.titleMolecular-level effects on cell membrane models to explain the phototoxicity of gold shell-isolated nanoparticles to cancer cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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